前因素对不同检测原理的25羟维生素D检测结果的影响

The influence of previous factors on the detection results of 25⁃hydroxyvitamin D with different detection principles

目的 探讨分析前因素对不同检测原理的25羟维生素D[25(OH)D]检测结果的影响。方法 收集北京航天总医院2022年1月住院患者血清和血浆标本作为研究对象,采用免疫电化学发光法和液相色谱串联质谱法分别进行25(OH)D的检测,分析离心温度为室温和4℃、抗凝管和促凝管、采集后立即离心和常温储存8 h后离心、离心后常温和2~8℃储存8 h这4种因素对两种方法检测结果的影响。结果 使用免疫法检测25(OH)D,室温离心组、使用促凝管采血组、采集后常温放置8 h组的25(OH)D总量高于4℃离心组、抗凝管组、即刻离心组,差异有统计学意义(t=-7.398、2.852、3.506,P<0.05),离心后常温储存组的25(OH)D总量高于2~8℃储存组,差异无统计学意义(t=1.883,P>0.05)。使用质谱法检测25(OH)D,室温离心组、使用促凝管采血组、采集后常温放置8 h组、离心后常温储存组的25(OH)D总量高于4℃离心组、抗凝管组、即刻离心组、2~8℃储存组,差异均无统计学意义(t=-1.918、1.995、-1.499、-0.399,P>0.05)。结论 前因素对两种不同检测原理的25(OH)D检测结果的影响有差异,日常检测中应积极采取有效措施避免分析前因素对检测结果准确性的影响。

Objective To investigate the impact of pre-analytical factors on the results of 25-hy-droxyvitamin D detection using different detection.Methods The serum and plasma samples from inpatients at Beijing Aerospace General Hospital were collected as research objects.Immunoassay and liquid chromatogra-phy tandem mass spectrometry were used to detect 25-hydroxyvitamin D respectively.The effects of four fac-tors on the detection results of the two methods were analyzed:the centrifugation temperature was room tem-perature and 4℃,anticoagulant tube and procoagulant tube,centrifugation immediately after collection and af-ter storage at room temperature for 8 hours,storage at room temperature and 2-8℃for 8 hours after centrifuga-tion.Results The immunoassay method was used to detect 25(OH)D levels.The results showed that the room temperature centrifugation group,blood collection group with procoagulant tube and the group where blood was left at room temperature for 8 hours had higher levels compared to the 4℃centrifugation group,8 anticoagulant tube group,and immediate centrifugation group.These differences were statistically significant(t=-7.398,2.852,3.506,P<0.05).Additionally,the results of the group stored at room temperature were higher than those stored at 2-8℃,but this difference was not statistically significant(t=1.883,P>0.05).Mass spectrometry was used to detect 25(OH)D levels.Similar to the immunoassay results,the levels in the room temperature centrifugation group,blood collection group with with a procoagulant tube,group where blood was left at room temperature for 8 hours,and the storage group at room temperature after centrifugation were higher than those in the 4℃centrifugation group,anticoagulant tube group,immediate centrifugation group,and storage group at 2~8℃.However,there were no significant differences found(t=-1.918,-1.995,-1.499,-0.399,P>0.05).Conclusion The influence of pre-factors on the 25-hydroxyvitamin D test results of two different test principles varies.It is important to implement effective measures to prevent pre-factors from af-fecting the accuracy of test results during daily testing.