卵巢癌患者血清LncRNA SNHG11和HIF-1α水平表达与癌组织血管生成拟态的关系研究

Study on the Relationship between Serum LncRNA SNHG11 and HIF-1αLevel Expression and Vasculogenic Mimicry of Cancer Tissues in Ovarian Cancer

目的探究卵巢癌(ovarian cancer)患者血清长链非编码RNA(long non-coding RNA,LncRNA)小核仁RNA宿主基因11(small nucleolar RNA host gene 11,SNHG11)和缺氧诱导因子1α(hypoxia inducible factor-1α,HIF-1α)表达与组织血管生成拟态(vasculogenic mimicry,VM)的关系。方法以2019年10月~2023年1月于唐山市妇幼保健院收治的116例卵巢癌患者为研究对象,根据是否形成VM将卵巢癌患者分为VM组(n=51)和无VM组(n=65),另选取同期进行健康体检者50例为对照组。采用实时荧光定量PCR法(real-time quantitative PCR,qPCR)检测卵巢癌患者及对照组血清LncRNA SNHG11和HIF-1α表达水平;Spearman相关性分析LncRNA SNHG11,HIF-1α与VM形成之间的关系;通过受试者工作特征(receiver operating characteristic,ROC)曲线分析LncRNA SNHG11,HIF-1α及联合检测对卵巢癌患者形成VM的诊断价值。结果与对照组相比,卵巢癌患者VM组和无VM组血清LncRNA SNHG11(3.01±0.88,2.21±0.68 vs 12±0.35),HIF-1α(2.16±0.67,1.60±0.44 vs 1.01±0.31)水平均显著升高(t=12.136,9.006;19.890,16.591),且VM组患者血清LncRNA SNHG11,HIF-1α水平均显著高于无VM组(t=8.957,8.595),差异有统计学意义(均P<0.05);LncRNA SNHG11,HIF-1α表达及VM的形成与年龄、组织类型无关(t=1.036,0.976,0.218;1.254,1.390,0.368,均P>0.05),而与肿瘤大小、FIGO分期、淋巴结转移及病理分级有关(t=5.351,5.186,13.264;5.465,5.227,10.898;6.063,6.016,5.374;4.030,5.871,5.509,均P<0.05);Spearman相关性分析显示,LncRNA SNHG11,HIF-1α与VM的生成均呈显著正相关(r=0.560,0.494,均P<0.05);ROC曲线结果显示,血清LncRNA SNHG11,HIF-1α单独诊断卵巢癌患者形成VM的曲线下面积(area under the curve,AUC)分别为0.860,0.824,其敏感度分别为80.4%,75.6%,特异度分别为58.9%,51.9%;两者联合诊断卵巢癌患者形成VM的AUC为0.941,其敏感度、特异度分别为92.2%,79.9%。结论LncRNA SNHG11和HIF-1α的异常表达与卵巢癌患者形成VM密切相关,两者可作为潜在判断VM的生物学指标。

Objective To investigate the relationship between the expression of long non-coding RNA(LncRNA)small nucleolar RNA host gene 11(SNHG11)and hypoxia inducible factor(HIF)-1αand angiogenesis mimicry(VM)in ovarian cancer.Methods A total of 116 ovarian cancer patients admitted to Tangshan Maternal and Child Health Care Hospital from October 2019 to January 2023 were regarded as the research subjects.Based on whether VM had formed,ovarian cancer patients were grouped into VM group(n=51)and non VM group(n=65).Another 50 partients who underwent health examinations during the same period were regarded as the control group.Real-time fluorescence quantitative PCR(qPCR)was applied to detect the expression levels of LncRNA SNHG11 and HIF-1αin serum of ovarian cancer patients and control groups.Spearman correlation was applied to detect the relationship between LncRNA SNHG11,HIF-1α,and VM formation.The diagnostic value of LncRNA SNHG11,HIF-1α,and their combined detection in the formation of VM in ovarian cancer patients was analyzed using the receiver operating characteristic(ROC)curve.Results Compared with the control group,the levels of serum LncRNA SNHG11(3.01±0.88,2.21±0.68 vs 1.12±0.35)and HIF-1α(2.16±0.67,1.60±0.44 vs 1.01±0.31)in ovarian cancer patients with VM group and non VM group were increased(t=12.136,9.006;19.890,16.591,all P>0.05),the levels of serum LncRNA SNHG11 and HIF-1αin the VM group were obviously higher than those in the non VM group(t=8.957,8.595),and the differences were statistically significant(all P<0.05).The expression of LncRNA SNHG11,HIF-1α,and the formation of VM were not related to age and tissue type(t=1.036,0.976,0.218;1.254,1.390,0.368,all P>0.05),but were related to tumor size,FIGO staging,lymph node metastasis,and pathological grading(t=5.351,5.186,13.264;5.465,5.227,10.898;6.063,6.016,5.374;4.030,5.871,5.509,all P<0.05).Spearman correlation analysis showed that there were obvious positive correlations between LncRNA SNHG11,HIF-1α,and VM generation(r=0.560,0.494,all P<0.05).ROC curve results showed that the areas under the curve(AUCs)of serum LncRNA SNHG11 and HIF-1αfor diagnosing VM formation in ovarian cancer patients were 0.860 and 0.824,respectively,with sensitivity of 80.4%and 75.6%,specificity of 58.9%and 51.9%,respectively.The AUC of VM formation in ovarian cancer patients diagnosed by the combination of the two was 0.941,with sensitivity and specificity were 92.2%and 79.9%,respectively.Conclusion The abnormal expressions of LncRNA SNHG11 and HIF-1αwere closely related to the formation of VM in ovarian cancer patients,and both may serve as potential biological indicators for judging VM.