GC-MS/MS法检测食品中环氧乙烷的代谢物2-氯乙醇

Determination of 2-Chloroethanol,a Metabolite of Ethylene Oxide in Food by GC-MS/MS

本文建立了气相色谱-质谱联用仪检测食品中环氧乙烷的代谢物2-氯乙醇残留量的分析方法。样品经分散固相萃取前处理,脉冲进样,外标法定量。结果表明,2-氯乙醇在50~800 ng·mL^(-1)时线性关系良好,相关系数R2为0.9994;检出限为50 ng·mL^(-1),定量限为150 ng·mL^(-1);添加水平为2 mg·kg^(-1)时,平均回收率为89.5%~104.9%。该方法前处理操作便捷,耗材价格低,准确度较高,满足大多数实验室需求,可以用于食品中环氧乙烷的代谢物2-氯乙醇的测定。

This article establishes an analytical method for detecting residual amounts of 2-chloroethanol,a metabolite of ethylene oxide,in food using gas chromatography-mass spectrometry.The sample was pre-treated with dispersion solid-phase extraction,pulse injection,and quantified using external standard method.The results showed that there was a good linear relationship between ethylene oxide and 2-chloroethanol at 50~800 ng·mL^(-1),with a correlation coefficient R2 of 0.9994;the detection limit is 50 ng·mL^(-1),and the quantification limit is 150 ng·mL^(-1);when the addition level is 2 mg·kg^(-1),the average recovery rate is 89.5%to 104.9%.This method has convenient pre-treatment operation,low consumable cost,high accuracy,and meets the needs of most laboratories.It can be used for the determination of ethylene oxide and its metabolite 2-chloroethanol in food.