英国红芸豆抗氧化肽对氧化应激斑马鱼的保护作用研究

The Protective Effect of British Red Kidney Bean Antioxidant Peptides on Oxidatively Stressed Zebrafish

为探究英国红芸豆抗氧化肽组分(BRKBPAPC)的体内抗氧化作用,利用2,2′-偶氮二异丁基脒二盐酸盐诱导建立氧化应激斑马鱼模型,研究BRKBPAPC对氧化应激斑马鱼的影响。结果表明:BRKBPAPC能显著提高(P<0.05)斑马鱼体内过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力和总抗氧化能力(T-AOC),显著降低(P<0.05)丙二醛(MDA)含量;同时BRKBPAPC还具有调节斑马鱼脂代谢的能力,随着处理浓度的增加斑马鱼脂代谢指标逐渐恢复正常水平;高剂量BRKBPAPC可使斑马鱼行为轨迹增多,高速运动次数、时间、距离显著上升。高剂量抗氧化肽处理21 d时,CAT、SOD、T-AOC、GSH-Px分别增至(28.29±3.29)、(126.59±6.39)、(2.07±0.18)、(84.75±7.77)U/mg prot,MDA含量降至(3.35±0.29)nmol/mg prot。由此可见,BRKBPAPC具有较好的体内抗氧化作用及脂代谢调节能力。

To investigate the in vivo antioxidant effect of British red kidney bean antioxidant peptide components(BRKBPAPC),an oxidative stress zebrafish model was established using 2,2′-azobis(isobutyl)amidine dihydrochloride induction to investigate the effect of BRKBPAPC on oxidatively stressed zebrafish.The results indicated that BRKBPAPC significantly increased(P<0.05)the activity of catalase(CAT),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and total antioxidant capacity(T-AOC),and significantly decreased(P<0.05)the malondialdehyde(MDA)content in zebrafish,BRKBPAPC also had the ability to regulate zebrafish lipid metabolism,with the increase of treatment concentration zebrafish lipid metabolism index gradually restored to normal level;high dose of BRKBPAPC could make zebrafish behavior trajectory increased,the number of high-speed movement,time,distance significantly increased.At 21 d of high-dose antioxidant peptide treatment,CAT,SOD,T-AOC,and GSH-Px increased to(28.29±3.29),(126.59±6.39),(2.07±0.18),(84.75±7.77)U/mg prot,MDA content decreased to(3.35±0.29)nmol/mg prot,showing that BRKBPAPC had good in vivo antioxidant effect and lipid metabolism regulation ability.