血浆SDC2基因甲基化检测对胃癌诊断的应用价值

The value of methylated DNA SDC2 detection in plasma for diagnosis of gastric cancer

目的探讨在血浆中检测SDC2基因启动子甲基化在胃癌诊断中的应用价值。方法收取2019年3月至2021年9月共211例血浆标本,其中胃癌患者94例作为胃癌组,同期健康体检者117名作为健康对照组,采用基于甲基化特异性PCR的方法检测血浆中甲基化的SDC2。结果SDC2检测在Ⅰ期、Ⅱ期、Ⅲ期、Ⅳ期胃癌中的阳性率分别为:27.8%(5/18)、27.3%(6/22)、47.8%(11/23)、57.1%(4/7),敏感度为38.3%(36/94),特异度为94.9%(111/117),诊断胃癌的AUC为0.645。甲基化状态与患者年龄、性别、肿瘤部位、肿瘤大小、分化程度及淋巴结转移之间不存在相关性(P均>0.05)。但随着肿瘤分期增加,SDC2甲基化检测对诊断胃癌的敏感度也逐渐增加。结论血浆SDC2甲基化检测对胃癌的诊断有一定的临床价值。

Objective To investigate the value of detecting SDC2 gene promoter methylation of plasma in the diagnosis of gastric cancer.Methods Plasma samples of 211 patients from Mar.2019 to Sep.2019 were enrolled and allocated to gastric cancer group(n=94)and healthy control group(n=117).Methylated SDC2 was detected using a methylation-specific PCR-based method.Results The sensitivity of SDC2 in stageⅠ,Ⅱ,ⅢandⅣgastric cancers was 27.8%(5/18),27.3%(6/22),47.8%(11/23)and 57.1%(4/7),the overall sensitivity was 38.3%(36/94),the specificity was 94.9%(111/117),and the AUC for diagnosis of gastric cancer was 0.645.There was no correlation between methylation status with the patient age,gender,tumor site,tumor size,degree of differentiation and lymphatic metastasis(all P>0.05).However,with the increase of tumor stage,the positive rate of SDC2 methylation also increased.Conclusion The detection of plasma SDC2 methylation has certain clinical value in the diagnosis of gastric cancer.