长梗瑞香中黄酮类化学成分研究

Study on Flavonoids from Daphne pedunculata

目的研究采自云南省元阳县长梗瑞香Daphne pedunculata中的黄酮类化学成分。方法采用正相、反相硅胶柱色谱、Sephadex LH-20凝胶柱色谱、半制备液相色谱等色谱方法进行分离纯化,运用核磁共振和质谱等波谱方法鉴定化合物的结构。结果从长梗瑞香95%乙醇提取物中分离得到21个黄酮化合物,其中双黄酮化合物12个。分别鉴定为异狼毒素(1)、新狼毒素A(2)、新狼毒素B(3)、荛花醇B(4)、毛瑞香素D2(5)、荛花醇A(6)、genikwanol A(7)、瑞香黄烷B(8)、wikstaiwanone B(9)、wikstaiwanone A(10)、瑞香黄烷G(11)、芹菜素(12)、木犀草素(13)、芫花素(14)、紫云英苷(15)、芹菜素-7-O-葡萄糖苷(16)、山柰酚(17)、木犀草苷(18)、刺槐素(19)、瑞香黄烷F(20)、5-甲氧基-芹菜素-7-O-β-D-葡萄糖苷(21)。结论从长梗瑞香95%乙醇提取物中分离得到21个黄酮化合物中,除化合物12~14外,其余均为首次从长梗瑞香中分离得到。该研究结果丰富了长梗瑞香植物黄酮的化学多样性,并为其深入研究及利用提供重要的基础和科学依据。

Objective To study the flavonoids from Daphne pedunculata H.F.Zhou ex C.Y.Chang.Methods The compounds were isolated and purified by silica gel column chromatography,Sephadex LH-20 gel column chromatography and semi-HPLC,and their structures were elucidated by NMR and mass spectrometry.Results 21 flavonoids were isolated from the 95%ethanol extract of Daphne pedunculata,including 12 biflavones.They were identified as isochamaejasmin(1),neocohamaejasmin A(2),neocohamaejasmin B(3),wikstrol B(4),daphnodorin D2(5),wikstrol A(6),genikwanol A(7),daphnodorin B(8),wikstaiwanone B(9),wikstaiwanone A(10),daphnodorin G(11),apigenin(12),luteolin(13),genkwanin(14),astragalin(15),apigenin-7-O-glucoside(16),kaempferol(17),luteolin-7-O-glucoside(18),acacetin(19),daphnodorin F(20),5-methoxy-apigenin-7-O-β-D-glucoside(21).Conclusion Among the 21 flavonoid compounds isolated from the 95%ethanol extract of Daphne pedunculata,except compounds 12~14,the others were isolated from Daphne pedunculata for the first time.The research results enrich the chemical diversity of flavonoids in Daphne pedunculata,and provide an important foundation and scientific basis for its in-depth research and utilization.