TUG1通过调控EZH2促进胃癌细胞侵袭转移的机制研究

Mechanism of TUG1 promoting invasion and metastasis of gastric cancer cells by regulating EZH2

目的:探讨牛磺酸上调基因1(TUG1)在胃癌细胞侵袭转移中的作用及其调控机制。方法:TCGA数据库分析TUG1在胃癌组织中的表达且与患者预后的关系;qRT-PCR检测胃癌组织和细胞中TUG1表达;细胞转染构建TUG1过表达后敲减细胞系;Transwell实验观察TUG1过表达和敲减对胃癌SGC-7901细胞迁移和侵袭能力的影响;Western Blot检测TUG1过表达和敲减对胃癌SGC-7901细胞中EZH2/STAT3信号通路活性的影响。结果:TUG1在胃癌组织和细胞中表达上调(P<0.01),与患者的预后呈负相关(P<0.01),而与其病理分期和淋巴转移呈正相关(P<0.05)。Transwell实验结果显示,上调TUG1表达促进胃癌细胞的迁移和侵袭(P<0.01)。Western Blot结果显示,上调TUG1表达可促进EZH2表达,并激活STAT3信号通路(P<0.01)。结论:TUG1通过激活EZH2/STAT3信号通路促进胃癌细胞的侵袭转移。

Objective:To explore the role and regulatory mechanism of taurine upregulated gene 1(TUG1)in the invasion and metastasis of gastric cancer cells.Methods:TCGA database analysed TUG1 expression in gastric cancer tissue and its relationship with patient prognosis.TUG1 expression in gastric cancer tissues and cells was detected by qRT-PCR.TUG1 overexpressed knockdown cell line was constructed by cell transfection.Transwell experiment observed the effects of TUG1 overexpression and knockdown on the migration and invasion ability of gastric cancer SGC-7901 cells.Western Blot detected the effects of TUG1 overexpression and knockdown on the activity of the EZH2/STAT3 signaling pathway in gastric cancer SGC-7901 cells.Results:qRT-PCR and TCGA database showed that TUG1 expression was up-regulated in gastric cancer tissues and cells(P<0.01),and was negatively correlated with prognosis of gastric cancer patients(P<0.01),but positively correlated with pathological stages and lymphatic metastasis of gastric cancer patients(P<0.05).Transwell assay showed that upregulation of TUG1 promoted the migration and invasion of gastric cancer cells(P<0.01).Western Blot result showed that upregulation of TUG1 promoted EZH2 expression and activated STAT3 signaling pathway(P<0.01).Conclusion:TUG1 promotes the invasion and metastasis of gastric cancer cells by activating EZH2/STAT3 signaling pathway.