甲基化蛋白7B抗体在成人急性髓系白血病中的表达及临床意义

Expression and clinical significance of METTL7B in adult acute myeloid leukemia

目的 基于癌症基因组图谱(TCGA)和基因表达综合数据库(GEO)数据分析甲基化蛋白7B抗体(METTL7B)在成人急性髓系白血病(AML)中的差异表达及临床意义,预测其在AML中可能的作用机制。方法 采用在线生物信息学数据库整合TCGA和GEO数据分析METTL7B在成人AML组织中的差异表达与生存预后,以及其与药物反应和免疫细胞浸润的相关关系。对METTL7B基因进行功能富集分析。结果与健康成人组织相比,成人AML组织中METTL7B表达水平升高(P<0.05)。METTL7B高表达与成人AML患者较短的总生存期和无事件生存期呈正相关(P<0.05)。METTL7B高表达与组蛋白脱乙酰基酶1(HDAC1)抑制剂耐药和AML中的单核细胞/巨噬细胞(尤其是M2巨噬细胞)免疫浸润呈正相关(P<0.05)。METTL7B基因参与调控巨噬细胞活化生物过程,参与巨噬细胞标记和调控肿瘤微环境中的免疫细胞与microRNAs相互作用。结论 METTL7B表达在成人AML组织中显著上调。METTL7B高表达与成人AML患者的不良预后相关,可作为AML新的预后生物标志物。METTL7B基因可能通过介导HDAC1抑制剂耐药和M2巨噬细胞免疫浸润参与AML疾病进展。

Objective To investigate the differential expression and its clinical significance of METTL7B in adult acute myeloid leukemia(AML)based on the Cancer Genome Atlas(TCGA)and GEO data,and further predict its possible mechanism in AML.Methods The differential expression of METTL7B and survival progno-sis in adult AML tissues,as well as its association with drug response and immune cell infiltration were analyzed by integrating TCGA and GEO data from online bioinformatics databases.Functional enrichment analysis of MET-TL7B gene were performed.Results Compared with normal adult tissues,the expression of METTL7B in adult AML tissues was significantly increased(P<0.05).The high expression of METTL7B was significantly positively correlated with shorter overall survival and event-free survival in adult AML patients(P<0.05).The high expres-sion of METTL7B was significantly positively correlated with HDAC1 inhibitor resistance and the immune infiltra-tion of monocytes or macrophages(especially M2 macrophages)in AML(P<0.05).The METTL7B gene played a crucial role in regulating the biological processes of macrophage activation,and was involved in macrophage la-beling and regulating the interaction between immune cells and microRNAs in the tumor microen-vironment.Conclusions The expression of METTL7B is significantly up-regulated in adult AML tissues.Elevated expression of METTL7B is associated with poor prognosis in adult AML patients,suggesting its potential as a novel prognostic biomarker for AML.The METTL7B gene may participate in the progression of AML by mediating HDAC1 inhi-bitor resistance and M2 macrophage immune infiltration.