基于网络药理学研究培元化瘀解毒方治疗胃癌的作用机制

Research on the mechanism of Peiyuan Huayu Jiedu formula in the treatment of gastric cancer based on network pharmacology

目的探讨培元化瘀解毒方治疗胃癌的网络药理学作用机制。方法利用中医药数据库和文献检索筛选培元化瘀解毒方的生物活性成分,预测其作用靶点,从疾病数据库检索胃癌相关靶点,构建药效分子-靶点网络及蛋白质-蛋白质相互作用(PPI)网络,然后进行KEGG分析、GO富集分析。结果检索获得培元化瘀解毒方中药的有效活性成分206种,药效分子靶点基因211个,有对应靶点信息的有效成分132个,对应关系1131个;检索得到胃癌靶点184个;通过PPI对药物靶点进行扩增得到3592个直接或间接靶点,与疾病靶点取交集得到144个靶点。PPI分析表明,排名靠前的靶点与胃癌密切相关,富集分析也显示这些靶点在癌症通路中被富集。结论本研究初步揭示了培元化瘀解毒方在胃癌治疗中的有效成分、潜在靶点及可能的作用机制,为中西医结合治疗胃癌提供参考。

Objective To explore the network pharmacology mechanism of Peiyuan Huayu Jiedu formula in the treatment of gastric cancer.Methods The biological active ingredients of Peiyuan Huayu Jiedu formula were screened by using traditional Chinese medicine database and literature retrieval,and its action targets were predicted.The related targets of gastric cancer were retrieved from the disease database,and the pharmacodynamic molecule-target network and protein-protein interaction(PPI)network were constructed,and then KEGG analysis and GO enrichment analysis were carried out.Results A total of 206 effective active components,211 pharmacodynamic molecular target genes,132 effective components with corresponding target information and 1131 corresponding relationships were obtained.184 targets of gastric cancer were retrieved.A total of 3592 direct or indirect targets were obtained by PPI amplification of drug targets,and 144 targets were obtained by intersection with disease targets.PPI analysis showed that the top-ranked targets were closely related to gastric cancer,and enrichment analysis also showed that these targets were enriched in cancer pathways.Conclusion This study preliminarily revealed the effective components,potential targets and possible mechanisms of Peiyuan Huayu Jiedu formula in the treatment of gastric cancer,and provided a reference for the treatment of gastric cancer with integrated traditional Chinese and western medicine.