Box-Behnken Design响应面法优化银杏叶提取物醇提水沉工艺

Optimization of Alcohol Extraction and Water Precipitation Process of Ginkgo Biloba Extract by the Box-Behnken Design-Response Surface Methodology

目的优化银杏叶提取物的醇提水沉工艺。方法在单因素试验基础上,以总黄酮醇苷转移率、萜类内酯转移率及干物质得率为综合评价指标,通过Box-Behnken Design(BBD)响应面法对液料比、提取时间、提取次数、提取液浓缩倍数、加水倍数、冷沉时间进行考察。结果醇提水沉最佳工艺为液料比8∶1(V/m),提取时间1.0 h,提取次数3次,浓缩倍数3倍,加水倍数3倍,冷沉时间28.0 h。结论BBD响应面法优化得到的银杏叶提取物醇提水沉工艺科学、合理、稳定、可行。

Objective To optimize the alcohol extraction and water precipitation process of Ginkgo biloba extract(GBE).Methods Based on the one-factor experiment,the transfer rate of total flavonol glycosides,and terpene lactones,and the dry matter yield were taken as comprehensive evaluation indicators,the liquid-solid ratio,extraction time,extraction times,concentration times of extraction liquid,water addition times and cryoprecipitation time were comprehensively investigated by the Box-Behnken Design(BBD)-response surface methodology(RSM).Results The optimal alcohol extraction and water precipitation process was as follows:the liquid-solid ratio was 8∶1(V/m),the extraction time was 1.0 h,the extraction times were three times,the concentration times were three times,the water addition times was three times,and the cryoprecipitation time was 28.0 h.Conclusion The optimal alcohol extraction and water precipitation process of GBE was designed by BBD-RSM,which is scientific,reasonable,stable and feasible.