纤毛蛋白TMEM138的保守性及拓扑结构

The conservation and topological structure of ciliary protein TMEM138

TMEM138是一种纤毛蛋白,其缺失会导致系统发育性纤毛病-Joubert综合征。为解析其致病的分子机制,本文对TMEM138进行了保守性分析和拓扑结构研究。选取8个代表性物种分析TMEM138蛋白一级、二级和三级结构的保守性,确定了TMEM138的跨膜结构域最为保守,是实现其功能的重要结构基础。通过构建TMEM138体外过表达载体并结合活细胞染色技术,首次验证了TMEM138的膜定位和其拓扑学结构,该结论可为TMEM138的研究提供进一步的帮助。

TMEM138 is a ciliary protein.Absence of TMEM138 can cause phylogenetic ciliopathy-Joubert syndrome.In order to analyze the molecular mechanism of TMEM138,the conservative analysis and topological structure of TMEM138 were studied.Eight representative species were selected to analyze the conserved primary,secondary and tertiary structure of TMEM138 protein,and determined that the transmembrane domain of TMEM138 is the most conserved,which is an important structural basis for realizing its function.By constructing the in vitro overexpression vector of TMEM138 and combining with the live cell staining technique,we verified the membrane localization and topological structure of TMEM138 for the first time.This conclusion can provide further help for the study of TMEM138.