吉祥草多糖的单糖组成及抗氧化活性分析

Composition Analysis and Antioxidant Activity of Polysaccharide from Reineckia Carnea(Andr.)Kunth

优化吉祥草多糖(RCP)提取工艺,探讨RCP的单糖组成和体外抗氧化活性。以吉祥草全草为原料,经烘干、粉碎、脱脂、脱色等前处理,在单因素基础上,利用正交试验优化RCP的提取工艺,采用高效液相色谱仪(HPLC)测定单糖组成,并探讨RCP体外抗氧化活性。RCP提取最佳工艺为料液比1∶40(g∶mL),提取温度100℃,提取时间60 min,提取次数3次,此条件下RCP提取率为17.23%±0.78%。经HPLC分析表明,RCP主要由8种单糖组成,分别是半乳糖醛酸41.90%±0.01%、半乳糖9.14%±0.01%、阿拉伯糖4.20%±0.03%、鼠李糖1.99%±0.02%、葡萄糖1.98%±0.01%、甘露糖1.06%±0.01%、葡萄糖醛酸0.49%±0.01%。体外抗氧化活性分析表明,RCP具有较强的体外抗氧化能力,在1.0 mg/mL质量浓度时,DPPH自由基、羟自由基、O_(2)^(-)·自由基、模拟胃液NO_(2)^(-)自由基的清除率各为91.49%±2.76%,29.33%±2.09%,42.37%±2.65%,25.17%±1.06%,IC_(50)值分别为0.08,1.67,1.11,2.42 mg/mL。吉祥草有一定的抗氧化能力,本研究有利于吉祥草多糖在功能性食品、保健品和药物等领域的开发利用,为吉祥草的开发利用提供理论依据。

To optimize the extraction process of RCP and to explore the monosaccharide composition and antioxidant activity of RCP in vitro.Using Reineckia carnea(Andr.)Kunth whole grass as raw material,after drying,crushing,degreasing,decolorization and other pre-treatments,on the basis of single factors,orthogonal experiments were used to optimize the extraction process of RCP.HPLC was used to determine the composition and content of monosaccharides,and the antioxidant activity of polysaccharide in vitro was discussed.The best extraction process of RCP was 1∶40(g/mL^(-1)),temperature 100℃,time 60 min,and extraction three times,under which the polysaccharide yield was 17.23%±0.78%.HPLC analysis showed that the polysaccharide were mainly composed of eight monosaccharides,namely galacturonic acid 41.90%±0.01%,galactose 9.14%±0.01%,arabinose 4.20%±0.03%,rhamnose 1.99%±0.02%,glucose 1.98%±0.01%,mannose 1.06%±0.01%,glucuronic acid 0.49%±0.01%.The scavenging rates of DPPH radical,hydroxyl radical,O_(2)^(-)radical and simulated gastric juice NO_(2)^(-)radical were 91.49%±2.76%,29.33%±2.09%,42.37%±2.65%,25.17%±1.06% at a concentration of 1.0 mg/mL,and IC_(50) values were 0.08,1.67,1.11 and 2.42 mg/mL,respectively.It has certain antioxidant capacity,which is conducive to the development and utilization of polysaccharide in the fields of functional foods,health products and drugs,and provides a theoretical basis for the development and utilization of Reineckia carnea(Andr.)Kunth.