红笛鲷免疫器官发育组织学观察

Histological Observation of Ontogeny of Immune Organs in Red Striped Snapper(Lutjanus sanguineus)

【目的】研究红笛鲷(Lutjanus sanguineus)免疫器官发育,为其健康养殖和疾病预防提供理论基础。【方法】从红笛鲷卵出膜开始取样,至出膜62 d,通过石蜡连续切片,H-E染色,进行红笛鲷3个主要免疫器官胸腺、头肾、脾脏发育的组织学研究。【结果与结论】红笛鲷出膜1 d时出现前肾,3 d肾管之间可见造血干细胞;出膜18 d,胸腺和头肾间出现细胞桥,头肾淋巴化;出膜62 d,肾小球仍存在于头肾中,表明头肾既是淋巴器官也是泌尿器官。红笛鲷出膜8 d时出现脾脏原基和红细胞发育;出膜22 d时观察到淋巴细胞;出膜24 d时淋巴细胞显著变多。胸腺是最后一个出现的器官,红笛鲷出膜12 d时出现胸腺原基;15 d时发现胸腺淋巴化。

【Objective】The aim of this paper is to study the histology of the immune organs of red striped snapper(Lutjanus sanguineus),and provide a theoretical foundation for the healthy culture and disease prevention of L.sanguineus.【Method】The serial paraffin sections were prepared from larval and juvenile red striped snapper 1 to 62 days post hatch(dph).The paraffin sections were stained with haematoxylin and eosin(H-E)and examined by light microscopy to study the histology of the development of thymus,head kidney and spleen of red striped snapper.【Result and Conclusion】The pronephros appeared at the 1 dph,blast cell at the 3 dph,a cell bridge between thymus and head kidney and lymphocytes at 18 dph,and the glomerulus still existed in the head kidney at 62 dph,indicating that renal was the lymphoid and urinary organ at that time.The progenitor spleen was found at 8 dph,little lymphocytes occasionally at 22 dph,more and more lymphocytes at 24 dph.The thymus was the last lymphoid organ to appear but showed a quick development.Progenitor thymus was found at 12 dph and lymphocytes were found at 15 dph.