基于MXene-MB-cDNA材料构建新型传感器方法用于镉离子检测

Construction of a New Sensing Method Based on MXene-MB-cDNA Material for Cadmium Ion Detection

本研究中,开发了一种新颖的基于过渡金属碳化物-亚甲基蓝-互补DNA(MXene-MB-cDNA)探针构建的竞争型电化学传感器,可用于重金属镉离子(Cd^(2+))的检测。该传感器一部分是基于MXene片层结构扩大MB负载率,用以放大电化学检测信号;另一部分以电极上修饰的Cd^(2+)Apt做为纽带连接MXene-MB-cDNA。当Cd^(2+)存在时,与cDNA竞争性结合Apt,引起双链结构变化,产生电化学信号,其最低检测限达到0.0034 mg·L^(-1),并在0.01至1.6 mg·L^(-1)范围内呈现良好的线性关系(R2=0.991)。相较传统方法,该方法更简单灵敏,为环境保护、食品安全、生物医药等研究方向中镉离子的检测提供了新的思路。

In this study,a novel competitive electrochemical sensor based on the transition metal carbide-methylene blue-complementary DNA probe(MXene-MB-cDNA)was developed for the detection of the heavy metal cadmium ion(Cd^(2+)).The sensor is partially based on the MXene layered structure to enhance the MB loading rate,which amplifies the electrochemical detection signal;The other part involves using a Cd^(2+)Apt modified on the electrode as a linker to connect MXene-MB-cDNA.When Cd^(2+)is present,it competes with cDNA for binding to Apt,causing a change in the double-stranded structure and generating an electrochemical signal.The lowest detection limit is 0.034 mg·L^(-1) and it shows good linearity in the range of 0.01 to 1.6 mg·L-1(R^(2)=0.991).Compared to traditional methods,this method is more simple and sensitive,providing a new approach for the detection of cadmium ions in research areas such as environmental protection,food safety,and biomedical sciences.