藏药喉毛花中3个环烯醚萜化合物的提取及其抑菌活性

Extraction of Three Eremophilane Sesquiterpenes from Tibetan Herbal Medicine Comastoma pulmonarium and Their Antibacterial Activity

对藏药喉毛花(Comastoma pulmonarium)中环烯醚萜的化学成分及其抑菌活性进行研究.使用硅胶、凝胶、树脂材料(MCI-GEL)及RP-HPLC色谱分析方法,从藏药喉毛花-丙酮(φ=70%)体系中提取分离得到3个环烯醚萜化合物,通过紫外光谱、红外光谱、质谱及核磁共振波谱等分析方法进行结构表征,并使用二倍稀释法对分离得到的化合物进行抑菌活性测定.结果表明,3个环烯醚萜化合物分别为(Z)-6-ethoxy-5-ethylidene-4,5,6,8-tetrahydro-1H,3H-pyrano[3,4-c]pyran-1-one(1);swerimilegenins H(2);swerimilegenins I(3),其中,化合物1为新化合物,化合物1—化合物3均具有一定的抑菌活性.

The chemical constituents of eremophilane sesquiterpenes in the Tibetan medicine Comastoma pulmonarium and their antibacterial activities were studied.Using chromatographic methods such as silica gel,gel,resin materials(MCI-GEL),and RP-HPLC,three eremophilane sesquiterpene compounds were extracted and isolated from the Comastoma pulmonarium-acetone(φ=70%)system.The structures were characterized by UV spectroscopy,infrared spectroscopy,mass spectrometry,and nuclear magnetic resonance spectroscopy.The antibacterial activity of the isolated compounds was assessed using the broth microdilution method.The results showed that the three eremophilane sesquiterpene compounds were identified as(Z)-6-ethoxy-5-ethylidene-4,5,6,8-tetrahydro-1H,3H-pyrano[3,4-c]pyran-1-one(1),swerimilegenins H(2),and swerimilegenins I(3),with compound 1 being a new compound.Compounds 1 to 3 all exhibited certain antibacterial activities.