幽门螺杆菌阴性的慢性萎缩性胃炎患者胃液微生物群特征分析

Characterization of gastric juice microbiota in Helicobacter pylori-negative patients with chronic atrophic gastritis

目的探究幽门螺杆菌阴性慢性萎缩性胃炎(CAGn)患者的胃液微生物群组成,寻找可能与CAGn发生相关的胃液微生物群。方法选取2020—2021年到兰州大学第一医院外科内镜中心接受胃镜检查的48例患者作为研究对象,其中幽门螺杆菌阴性慢性非萎缩性胃炎(CNAGn)患者21例,CAGn患者27例。收集胃液标本,使用16S rRNA三代全长测序技术测定分析两组患者的胃液微生物群组成。结果CAGn组的平均年龄高于CNAGn组[(52.33±12.20)岁vs.(40.67±7.95)岁,P=0.0004]。两组患者微生物α多样性及β多样性无明显差异,微生物群整体组成较为相似。组间差异显著性分析发现了CNAGn组显著富集的11个属和CAGn组显著富集的2个属。结论本研究筛选出CAGn患者的差异性非幽门螺杆菌微生物,这些差异微生物可能为CAGn患者的诊断和治疗提供参考。

Objective To explore the composition of the gastric juice microbiota in patients with Helicobacter pylori-negative chronic atrophic gastritis(CAGn),and to look for gastric juice microbiota that may be related to the occurrence of CAGn.Methods Forty-eight patients who underwent gastroscopy at the Surgical Endoscopy Center of the First Hospital of Lanzhou University from 2020 to 2021 were selected for the study,including 21 with Helicobacter pylori-negative chronic non-atrophic gastritis(CNAGn)and 27 with CAGn.Gastric juice samples were collected and the third-generation sequencing of the 16S rRNA was used to detect gastric microbial composition in the two groups.Results The average age of the CAGn group was significantly higher than that of the CNAGn group(52.33±12.20 vs.40.67±7.95,P=0.0004).There was no significant difference in the microbialα-andβ-diversity and the overall microbiota composition between the two groups.The significance analysis of the differences between the two identified 11 genera having been significantly enriched in the CNAGn group and 2 genera significantly enriched in the CAGn group.Conclusion In this study,the differential non-Helicobacter pylori microbiota of CAGn patients was screened,which may provide a reference for the diagnosis and treatment of CAGn patients.