广东汕尾汉族人群STR基因座遗传多态性分析

Genetic polymorphism analysis of STR loci in the Han population of Shanwei,Guangdong

目的:分析研究广东汕尾汉族人群中,D3S1358、TH01、D21S11、D18S51、Penta E、D5S818、D13S317、D7S820、D16S539、CSF1PO、Penta D、vWA、D8S1179、TPOX、FGA、D19S433、D12S391、D6S1043、D2S1338、D1S1656等20个常染色体STR基因座的遗传多态性。方法:采集广东汕尾地区汉族人群418例无血缘关系个体的FTA血卡,采用HEALTH Gene STRtyper-21G Plus试剂盒扩增DNA与分型,用GeneMapper软件分析,用SPSS和Modified-Powerstates软件统计等位基因频率和群体遗传学参数。结果:20个STR基因座等位基因的频率分布为0.001~0.571,个体识别率为0.771~0.984,多态信息含量为0.527~0.904,应用于二联体非父排除率为0.275~0.790,应用于三联体非父排除率为0.400~0.842,观察杂合度为0.586~0.897,杂合度期望值为0.587~0.912。基因型分布均符合Hardy-Weinberg平衡定律(P>0.05)。结论:汕尾地区汉族人群的20个常染色体STR基因座具有较高多态性,在人类学和群体遗传学中有较大的研究和应用价值,同样在亲权鉴定和个体识别中有较大价值。

Objective:To analyze the genetic polymorphisms of 20 autosomal STR loci in Shanwei population of Guangdong Province,including D3S1358,TH01,D21S11,D18S51,Penta E,D5S818,D13S317,D7S820,D16S539,CSF1PO,Penta D,vWA,D8S1179,TPOX,FGA,D19S433,D12S391,D6S1043,D2S1338 and D1S1656.Methods:The FTA blood cards of 418 unrelated individuals in Shanwei area of Guangdong Province were collected.The DNA was amplified and typed by health gene STRtyper-21g plus kit,analyzed by GeneMapper software,and the allele frequency and population genetic parameters were counted by SPSS and modified powerstates software.Results:The allele frequency distribution of 20 STR loci was 0.001-0.571,the power of discrimination was 0.771-0.984,the polymorphism information content was 0.527-0.904,the power of exclusion(duo-cases)was 0.275-0.790,the power of exclusion(tiros-cases)was 0.400-0.842,the observed heterozygosity was 0.586-0.897,and the expected heterozygosity was 0.587-0.912.The distribution of genotypes accorded with Hardy Weinberg equilibrium law(P>0.05).Conclusion:The 20 autosomal STR loci in shanwei population have high polymorphism,which is of great research and application value in anthropology and population genetics,as well as in parental identification and individual identification.