摘要
西番莲(Passiflora edulis)茎基腐病是危害西番莲生产最为严重的土传病害之一,其致病机理尚未完全明确,目前没有较为可靠的治疗方法,严重影响西番莲产业的发展.本研究探究西番莲茎基腐病发病与土壤理化性质和土壤细菌群落多样性的相关性,以期为西番莲茎基腐病的综合防治提供理论依据.通过对水田、旱地和山地3种地形西番莲果园的茎基腐病发病率进行调查以及对其土壤理化性质进行测定,并采用高通量测序对不同地形土壤进行细菌16S rDNA的V4高变区序列测序,分析了土壤细菌群落相关性和多样性指数.水田的西番莲茎基腐病发病率最高(80.67%),旱地次之(52.67%),山地最低(15.33%).水田土壤的pH值和电导率最高(7.25和49.43 us/cm),旱地次之(5.18和48.27 us/cm),山地最低(4.57和39.73 us/cm).土壤细菌群落丰度是水田最高,旱地次之,山地最低,且染病植株土壤的细菌群落丰度高于正常植株土壤;细菌群落多样性是旱地最高,山地次之,水田最低.聚类分析表明,不同样地土壤中细菌群落结构的组间差异较大,而水田组内相似度低,旱地和山地组内相似度高.西番莲果园茎基腐病发病率与土壤理化性质、地形因子和细菌的丰度和多样性有明显的相关性.
The stem base rot of Passiflora is one of the most serious soil-borne diseases endangering the production of Passiflora,which seriously affects the development of Passiflora industry.The mechanism of the disease is not fully clear and there is no reliable control method for this disease.To provid a theoretical basis for the comprehensive control of stem base rot of Passiflora,the correlation between the occurrence of stem base rot of Passiflora and soil physico-chemical properties and bacterial genetic diversity was explored in this study.The incidence of Passiflora base rot diseas in three topographical orchards,including paddy fields,dry farms and mountainous areas were investigated.The soil samples were collected from infected and normal plants in three Passiflora planting areas,respectively,and the physico-chemical properties,including pH value and electrical conductivity(EC)were measured.The V4 hypervariable regions of soil bacteria 16S rDNA were sequenced by high-throughput sequencing,and the relationship and diversity index of bacterial communities were analyzed.The incidence of Passiflora base rot is the highest in paddy fields(80.67%),followed by dry farms(52.67%),and mountainous areas(15.33%).The pH value and electrical conductivity of paddy soils were the highest(7.25 and 49.43 us/cm),followed by dry farms(5.18 and 48.27 us/cm)and mountainous area(4.57 and 39.73 us/cm).The bacterial community abundance is the highest in paddy fields,followed by dry farm and mountainous areas,and is higher in soils of infected plants than that of normal plants.The bacterial community diversity is the highest in dry farm,followed by mountainous areas and paddy fields.There is a large difference in the bacterial community structure between groups in soil samples from different sample plots,while the similarity is low within group of the paddy field,and is high within group of dry farms and group of mountain areas.The incidence rate of stem base rot in passionflower orchards is significantly correlated with physico-chemical properties,topographic factors and bacterial diversity in soils.
作者
郑良豹
谢敏霞
欧光敏
梁宪锵
谢秀凤
董安强
刘文
周玲艳
ZHENG Liangbao;XIE Minxia;OU Guangmin;LIANG Xianqiang;XIE Xiufeng;DONG Anqiang;LIU Wen;ZHOU Lingyan(College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;College of Tropical Agriculture and Forestry,Guangdong Agriculture Industry Business Polytechnic,Guangzhou 510507,China)
出处
《仲恺农业工程学院学报》
CAS
2023年第4期7-12,58,共7页
Journal of Zhongkai University of Agriculture and Engineering
基金
广东省科技计划项目(2015A02029172)
广东省科技特派员项目(2018KA1810329)
广东省教育厅2019年度普通高校服务乡村振兴重点领域科研专项项目(2019KZDZX2035)
广东省驻镇帮镇扶村农村科技特派员项目(粤科函农[2021]1056号)
广东省普通高校重点平台和重大科研项目(2019GKTSCX056)。