复合乳酸菌联合蒙脱石散对慢性腹泻患儿肠道菌群和氧化应激及炎性因子的影响

Effects of compound lactic acid bacteria combined with Montmorillonite powder on intestinal flora,oxidative stress and inflammatory factors in children with chronic diarrhea

目的探讨复合乳酸菌联合蒙脱石散对慢性腹泻患儿肠道菌群、氧化应激和炎性因子的影响,为该类患者的治疗提供参考。方法选取2020年6月至2022年8月我院门诊收治的400例慢性腹泻患儿作为研究对象,按照随机数表法分为观察组(予以复合乳酸菌联合蒙脱石散治疗)与对照组(仅予以复合乳酸菌治疗),各200例。对比2组患儿肠道菌群(双歧杆菌、乳杆菌、大肠埃希菌)和应激指标[丙二醛(MDA)、一氧化氮(NO)]、炎性因子[白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)]、胃肠道激素[胃泌素(GAS)、胃动素(MOT)、生长抑素(SSTI)]水平的变化。结果治疗后,观察组患儿肠道双歧杆菌、乳杆菌数量均高于对照组,而大肠埃希菌数量低于对照组(均P<0.05);同时观察组患儿MDA、NO、TNF-α、GAS、MOT水平均低于对照组,而IL-10、IFN-γ、SSTI水平均高于对照组(均P<0.05)。结论复合乳酸菌联合蒙脱石散治疗慢性腹泻的疗效显著,有利于调节患儿肠道菌群以及氧化应激、炎性因子水平。

Objective To explore the effects of compound lactic acid bacteria(LAB)combined with Montmorillonite powder on intestinal microecology,oxidative stress and inflammatory factors in children with chronic diarrhea,providing a reference for the treatment.Methods A total of 400 children with chronic diarrhea admitted in our hospital from June,2020 to August,2022 were randomly divided into observation group(given compound LAB combined with Montmorillonite powder)and control group(given compound LAB alone),with 200 cases in each group.The intestinal flora(Bifidobacterium,Lactobacillus,Escherichia coli),stress indicators[malondialdehyde(MDA),nitric oxide(NO)],inflammatory factors[interleukin 10(IL-10),tumor necrosis factorα(TNF-α),interferon-γ(IFN-γ)]and gastrointestinal hormones[gastrin(GAS),motilin(MOT),somatostatin(SSTI)]were compared between groups.Results After treatment,the counts of Bifidobacterium and Lactobacillus in the observation group were higher,while that of Escherichia coli was lower than those in the control group respectively(all P<0.05).The levels of MDA,NO,TNF-σ,GAS and MOT in the observation group were lower,while those of IL-10,IFN-γand SSTI were higher than those in the control group respectively(all P<0.05).Conclusion Compound lactic acid bacteria combined with Montmorillonite powder is effective in treating chronic diarrhea in children,which is beneficial to regulate intestinal microecology,oxidative stress and inflammatory factors.