江苏地区一株鲤浮肿病毒的检测和系统进化分析

Dentification and Phylogenetic Analysis of a Carp Edema Virus in Jiangsu Province

为及时掌握江苏省水生动物重大疫病流行趋势以便采取控制措施,应用国标或行标方法对江苏地区某养殖场的健康鲤鱼样品进行水生动物重大疫病监测,检测了锦鲤疱疹病毒(koi herpes virus, KHV)、鲤春病毒血症病毒(spring viremia of carp virus, SVCV)和鲤浮肿病毒(carp edema virus, CEV)。结果显示CEV阳性,系江苏省首次检出CEV。基于CEV P4a基因进行系统进化分析,结果显示该病毒与NCBI序列号为KY024583.1的CEV亲缘关系最近。该研究可为江苏省鲤浮肿病毒的流行传播规律探寻、疾病诊断和防控提供重要依据。

In order to timely grasp the epidemic trend of major aquatic animal diseases in Jiangsu Province and take control measures,national or industry standard methods were applied to monitor the major aquatic animal diseases in healthy carp samples from a breeding farm in Jiangsu Province.Koi herpes virus(KHV),spring virus of carp virus(SVCV),and carp edema virus(CEV)were detected.The results showed positive CEV,which is the first time CEV has been detected in Jiangsu Province.A phylogenetic analysis was conducted based on the CEV P4a gene,and it was found that the virus was most closely related to CEV with NCBI sequence number KY024583.1.The study can provide important basis for the epidemic and transmission patterns,disease diagnosis,and prevention and control of carp swelling virus in Jiangsu Province.