煤矸山优势植物两头毛根际耐镉真菌的分离鉴定

Isolation and Identification of Cd-tolerant Fungi from Rhizosphere Soil of Incarvillea arguta,a Dominant Plant from Coal Waste Pile

【目的】从煤矸山重金属污染土壤中分离纯化耐镉微生物,为进一步利用根际微生物修复土壤耐污染提供依据。【方法】利用含镉50 mg/L的PDA培养基从煤矸山优势植物两头毛根际土壤中分离纯化具有耐镉特性的菌株,并通过形态学和分子学方法对分离的菌株进行鉴定。【结果】从煤矸山优势植物两头毛根际土壤中共分离出9株真菌,分别为链格孢属(Alternaria sp.)1种、拟青霉属(Purpureocillium sp.)1种、透孢黑团壳属(Massarina sp.)2种、拟盘多毛孢属(Pestalotiopsis sp.)1种、被孢霉属(Mortierella sp.)1种、Setophoma sp.1种、柄孢壳菌属(Podospora sp.)1种和镰刀菌属(Fusarium sp.)1种。【结论】煤矸山优势植物两头毛根际土壤中含有丰富耐镉菌株资源。

【Objective】The Cd-tolerant microorganism was isolated and purified from heavy metal contaminated soil of coal waste pile to provide a reference for further utilization of rhizosphere microorganism remediating Cd-contaminated soil.【Method】The Cd-tolerant strains were isolated and purified using PDA medium containing 50 mg/L Cd from the rhizosphere soil of Incarvillea arguta,a dominant plant from coal waste pile,and then the isolated strains were identified through morphology and molecular methods.【Result】From the rhizosphere soil of I.arguta,a dominant plant from coal waste pile,a total of nine fungal strains were isolated,and they were respectively identified as one strain of Alternaria sp.,one strain of Purpureocillium sp.,two strains of Massarina sp.,one strain of Pestalotiopsis sp.,one strain of Mortierella sp.,one strain of Setophoma sp.,one strain of Podospora sp.and one stain of Fusarium sp..【Conclusion】The rhizosphere soil of dominant plant I.arguta from coal waste pile has abundant Cd-tolerant strain resource.