大豆种子蛋白亚基及11S/7S随种子发育的变化规律

Changes of Soybean Seed Protein Subunits and 11S/7S with Seed Development

为促进大豆蛋白品质改良,采用SDS-PAGE凝胶电泳对4份不同大豆品种种子发育过程中的蛋白亚基变化规律进行了分析。结果表明,在种子发育过程中发现蛋白含量高的品种中吉601(44.82%)在S4时期β亚基积累明显高于其他蛋白含量低的品种;不同大豆品种的11S组分的亚基在各个时期的积累明显不同,11S/7S比值低的两个品种中吉601(1.059)和吉林小粒豆(1.022),其11S组分中的Basic亚基在S3-S9时期的含量低于其他亚基的含量,而11S/7S比值高的两个品种黑河23(1.324)和绥农28(1.401),其11S组分中的Basic亚基和Acidic亚基在S3-S9时期含量高于其他亚基的含量,推测不同品种的11S/7S比值高低与Basic亚基在种子发育过程中的积累相关。

In order to promote the improvement of soybean protein quality,understand the dynamic changes of subunits of 11S and 7S components during the development of soybean seeds.in this study,SDS-PAGE was used to study the changes of protein subunits in four different soybean varieties during seed development.During seed development,it was found that the accumulation ofβsubunits in the variety Zhongji 601(44.82%)with high protein content in the S4 stage was significantly higher than that of other varieties with low protein content.The accumulation of subunits in the 11S component of different soybean varieties was significantly different at different stages.Among the two varieties with low 11S/7S ratios,Zhongji 601(1.059)and Jilin xiaolidou had a lower content of Basic subunits in the 11S component during the S3-S9 period compared to other subunits,while the two varieties with high 11S/7S ratios,Heihe 23(1.324)and Suinong 28(1.401),had a higher content of Basic and Acid subunits in the 11S component during the S3-S9 period compared to other subunits,which indicated the ratio of 11S/7S in different varieties was related to the accumulation of Basic subunits during seed development.