基于肠道菌群探讨黄诺玛苷对脾虚泄泻小鼠肠道功能的影响

Effect of flavanomarein on the intestinal function in mice with spleen deficiency diarrhea based on intestinal flora

目的基于肠道菌群探讨黄诺玛苷对脾虚泄泻小鼠肠道功能的影响。方法将小鼠随机分为空白组和造模组,造模组采用大黄水煎液灌服结合潮湿环境法建立脾虚泄泻模型;随后造模组随机分为模型组、黄诺玛苷组、阳性对照组,空白组和模型组灌服蒸馏水,给药组分别灌服相应药物。观察小鼠一般体征指标和结肠组织病理学变化,测定炎症因子水平,16S基因测序技术分析肠道菌群,利用1H-NMR技术检测小鼠粪便短链脂肪酸(SCFA)水平。结果黄诺玛苷会上调脾虚泄泻小鼠Bacteroidota、Muribaculaceae、Eubacterium-coprostanoligenesgroup等肠道优势有益菌群的丰度及肠道菌群多样性和丰富度,下调Firmicutes、Proteobacteria、Campilobacterota、Helicobacter、Ruminococcus-torques-group、Colidextribacter等肠道优势有害菌群的丰度,使肠道优势菌群丰度趋于空白组小鼠。肠道优势有益菌群跟脾虚泄泻小鼠血清白细胞介素6(IL-6)、肿瘤坏死因子-α(TNF-α)、血管活性肠肽(VIP)成负相关,与白细胞介素10(IL-10)、胃泌素(GAS)成正相关;肠道优势有害菌群跟之相反。结论黄诺玛苷可能是通过调节脾虚泄泻小鼠肠道微生态平衡,发挥抑制血清和肠道炎症、保护及修复肠黏膜、提升免疫功能、改变肠道SCFA水平作用,有效抑制小鼠脾虚泄泻的发生与恶化。

Objective To explore the effect of flavanomarein on the intestinal function in mice with spleen deficiency diarrhea based on intestinal flora.Methods Mice were randomly divided into a blank group and a modeling group,the modeling group was orally given rhubarb decoction combined with a humid environment method to establish a spleen deficiency diarrhea model.Subsequently,the modeling group was randomly divided into a model group,a flavanomarein group and a positive control group.The blank group and the model groups were given distilled water by gavage,while the treatment groups were given corresponding drugs by gavage.The general physical signs and pathological changes in the colon tissue of mice were observed,the levels of inflammatory factors were measured,the intestinal flora were analyzed with 16S gene sequencing technology,and the levels of short chain fatty acids(SCFA)in mouse feces were detected with ^(1)H-NMR technology.Results Flavanomarein upregulated the abundance of beneficial intestinal flora,the diversity and richness of intestinal flora in mice with spleen deficiency diarrhea,such as Bacteroidota,Muribalactaceae and Eubacterium coprostanogenes group.It also downregulated the abundance of harmful intestinal flora,such as Firmicutes,Proteobacteria,Campilobacterota,Helicobacter,Ruminococcus-torques-group and Colidextribacter,adjusting the abundance of dominant intestinal flora towards that of the blank group.The beneficial intestinal flora were negatively correlated with the serum inter leukin(IL)-6,tumor necrosis factor α(TNF-α),and vasoactive peptide(VIP)in mice with spleen deficiency diarrhea,but positively correlated with IL-10,and gastin(GAS).The opposite was true for the harmful intestinal flora.Conclusion Flavanomarein may regulate the intestinal flora balance in mice with spleen deficiency diarrhea,inhibit the serum and intestinal inflammation,protect and repair intestinal mucosa,enhance immune function,and change intestinal SCFA levels,effectively inhibiting the occurrence and deterioration of spleen deficiency diarrhea in mice.