金樱子HPLC多组分定量控制及化学计量学综合质量评价

Quantitative control of multi-component HPLC and comprehensive quality evaluation of chemometrics in Rosae Laevigatae Fructus

目的 对不同产地金樱子进行综合质量评价,为其道地研究提供依据。方法 收集8省16批次金樱子样品,采用高效液相色谱法同时检测金樱子中蔷薇酸、委陵菜酸、熊果酸、没食子酸、儿茶素、橙皮苷、芦丁、槲皮素、木犀草素、山柰酚、芹菜素和鞣花酸含量,建立金樱子多组分定量控制模式;采用主成分分析(PCA)、正交偏最小二乘法-判别分析(OPLS-DA)和熵权优劣解距离法(EW-TOPSIS)建立金樱子质量优劣评价模型,对其整体质量进行综合评价。结果12个成分在各自范围内线性关系良好,平均加样回收率96.94%~100.05%,RSD为0.72%~1.56%;16批样品聚为3类;鞣花酸、儿茶素、槲皮素和没食子酸可能是影响金樱子产品质量主要潜在标志物;EW-TOPSIS结果显示16批金樱子相对贴近度(Jb)为0.146 2~0.756 2,不同产地的金樱子品质差异较大。结论 所建立的方法操作便捷,结果准确,可作为金樱子多组分定量控制方法;化学计量学及EW-TOPSIS法客观全面,可用于金樱子的整体质量评价。

Objective To comprehensive quality evaluation of Rosae Laevigatae Fructus(RLF) from different producing areas,and to provide basis for studying the geoherbalism of RLF.Methods The 16 batches of RLF samples from eight provinces were collected.The high performance liquid chromatography(HPLC) method was used to simultaneous determine contents of euscaphic acid,tormentic acid,ursolic acid,gallic acid,catechin,hesperidin,rutoside,quercetin,luteolin,kaempferol,apigenin and ellagic acid,and the multi-components quantitative control mode of RLF was established.The principal component analysis(PCA) and orthogonal partial least squares-discriminant analysis(OPLS-DA) were used to screen potential markers affecting the quality of RLF.The quality evaluation model of RLF was established by using entropy weight-technique for order preference by similarity to an ideal solution(EW-TOPSIS) method,and the overall quality was evaluated comprehensively.Results The 12 components showed good linear relationships in their own ranges.The average recovery were 96.94%—100.05% with the RSDs of 0.72%—1.56%.Ellagic acid,catechin,quercetin and gallic acid,may be the main potential markers affecting the quality of RLF.The results of EWTOPSIS analysis showed that the quality evaluation closeness(Jb) of 16 batches of RLF were 0.146 2—0.756 2,The quality of RLF from different producing areas was very different.Conclusion The established HPLC method is convenient in operation and accurate in results,and can be used as a quantitative control method for multi-components of RLF.Chemometrics and EW-TOPSIS method are objective and comprehensive,and can be used for the overall quality evaluation of RLF.