禽腺病毒血清4型感染的LMH细胞mRNA表达谱分析

mRNA Expression Profile Analysis of LMH Cells Infected withFowl Adenovirus Serotype 4

禽腺病毒血清4型(FAdV-4)引起的禽肝炎-心包积液综合征已严重危害我国养禽业的发展,迫切需要深入了解FAdV-4的致病机制并制定有效的防控方案。论文旨在利用高通量测序技术分析禽腺病毒血清4型感染鸡肝癌(Leghorn male hepatocellular,LMH)细胞后差异表达的mRNA,以探究mRNA在FAdV-4感染过程中的作用及其调控机制。结果显示,与对照组相比,FAdV-4感染48 h共筛选到2604个上调的差异表达基因,2883个下调的差异表达基因。其中,GNB3、NECTIN4、SFRP4、FABP2、PLPP3、GALR1L和MBL 2等基因之前并没有被报道过与FAdV-4感染相关。通过GO和KEGG分析得知,差异表达基因主要富集在从细胞表面位点和信号受体结合至细胞周期调节、MAPK信号通路、细胞黏附分子作用等;其中,显著下调的差异基因还富集到多种代谢途径中,提示病毒感染会影响宿主细胞的代谢水平。本研究为深入解析FAdV-4感染宿主细胞的致病机制提供了相关的理论基础及科学依据。

Fowl adenovirus serotype 4(FAdV-4)caused hydropericardium syndrome-inclusion body hepatitis(HPS-IBH)has seriously endangered the development of the poultry industry in China.There is an urgent need to understand the pathogenic mechanism of FAdV-4 and to develop effective prevention and control programs.The aim of this study is to analyze the differentially expressed mRNA in Leghorn male hepatocellular(LMH)cells infected with FAdV-4 using high-throughput sequencing technology,and to explore the role and regulatory mechanism of mRNA in the process of FAdV-4 infection.Compared with the control group,2604 up-regulated genes and 2883 down-regulated genes were screened out at 48 h after FAdV-4 infection.Among them,GNB3,NECTIN4,SFRP4,FABP2,PLPP3,GALR1L and MBL2 have not been previously reported to be associated with FAdV-4 infection.GO and KEGG analysis showed that the differentially expressed genes were mainly enriched in cell surface sites and signal receptor binding to cell cycle regulation,MAPK signaling pathway,cell adhesion molecules,etc.Among them,the significantly down-regulated differential genes were also enriched in a variety of metabolic pathways,suggesting that viral infection can affect the metabolism level of host cells.This study provides a theoretical basis and scientific basis for further understanding the pathogenic mechanism of FAdV-4 infection in host cells.