许氏平鲉5-HT1A基因单核苷酸多态性与攻击行为表型相关性研究

ASSOCIATION OF SINGLE NUCLEOTIDE POLYMORPHISMS OF THE 5-HT1A GENE WITH DIFFERENT AGGRESSION PHENOTYPE IN SEBASTES SCHLEGELII

以许氏平鲉(Sebastes schlegelii)幼鱼为研究对象,旨在探究5-HT1A受体基因在攻击行为调控中的作用。首先,通过注射8-OH-DPAT(特异性5-HT1A受体激动剂)对许氏平鲉幼鱼的攻击行为进行了分析,评估了受体对幼鱼攻击行为的影响;其次,对不同攻击表型的许氏平鲉幼鱼进行了5-HT1A受体基因的多态性位点筛选,以期获得与许氏平鲉攻击行为显著相关的潜在SNP标记。结果显示:(1)8-OH-DPAT处理组幼鱼攻击行为的频率和时间显著低于对照组(P<0.05),但攻击潜伏期显著高于对照组(P<0.05);(2)在5-HT1Aα启动子区域中发现了7个与攻击表型差异显著相关的多态性位点(P<0.05),分别为SNP 1236、SNP 1245、SNP 1260、SNP1301、SNP 1302、SNP 1309和SNP 1330位点;在5-HT1Aβ启动子区域中发现了2个与攻击表型差异显著相关的多态性位点(P<0.05),分别为SNP 892和SNP 1147位点,但在5-HT1A基因CDS编码区并未发现与攻击差异表型显著相关的多态性位点(P>0.05)。综上所述,研究结果证明了5-HT1A受体的激活能够显著抑制许氏平鲉的攻击行为,筛选了与攻击性差异个体相关的5-HT1A受体基因SNP位点。

We used juvenile black rockfish(Sebastes schlegelii)to investigate the impact of the 5-HT1A receptor gene on aggression control in this study.The administration of 5-HT1A receptor agonist,8-OH-DPAT,was utilized to evaluate its impact on aggression among juvenile black rockfish.Subsequently,different aggression phenotypes within the juvenile groups were scrutinized for genetic variations in the 5-HT1A receptor gene,aiming to identify potential SNP markers correlated with aggression.Our findings showed that fish treated with 8-OH-DPAT exhibited significantly reduction in the frequency and duration of aggressive behavior compared to the control group(P<0.05).The latency period for aggression was significantly higher in the treated group(P<0.05).We discovered seven significant polymorphic sites linked to attack phenotypes in the 5-HT1Aαpromoter region(P<0.05):SNP 1236,SNP 1245,SNP 1260,SNP 1301,SNP 1302,SNP 1309,and SNP 1330.Additionally,two polymorphic sites were significantly associated with attack phenotypes in the 5-HT1Aβpromoter region(P<0.05):SNP 892 and SNP 1147.However,no significant polymorphic sites were identified in the coding region of the 5-HT1A gene in relation to attack phenotypes(P>0.05).In summary,the results demonstrate that activating the 5-HT1A receptor effectively inhibits aggressive behavior in black rockfish.Furthermore,we also identified SNP loci within the 5-HT1A receptor gene that are associated with different aggression profiles.