摘要
实验基于高通量测序技术,以健康(CTL组)、发育不良(DD组)和患歪头病(TD组)的棘胸蛙(Quasipaa spinosa)为研究对象,系统地探索宿主健康状态与皮肤微生物菌群组成、多样性及功能之间的潜在关系。所有样本经测序、质控及聚类获得2997条扩增子序列变体(ASVs),注释32个门,76个纲,167个目,259个科和402个属,其中变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidota)为棘胸蛙皮肤主要优势菌门(>1%),但丰度受宿主健康状态的影响。在属水平上筛选获得50个菌群标志物,其中23个(如乳杆菌(Lactobacillus)、德沃斯氏(Devosia)、希瓦氏菌(Shewanella)和Luteolibacter)在CTL组中相对丰度较高,提示棘胸蛙的健康状态;19个(如短稳杆菌(Empedobacter)、芽孢杆菌(Bacillus)和短螺旋体(Brevinema))在DD组中相对丰度较高,提示棘胸蛙发育不良的病症;8个(如伊丽莎白菌(Elizabethkingia)、柠檬酸杆菌(Citrobacter)和不动杆菌(Acinetobacter))在TD组中相对丰度较高,提示棘胸蛙歪头病的病症。多样性分析表明,TD组的多样性和均一度与CTL组和DD组相比显著降低,微生物菌群组成也表现出明显分离。功能预测表明,CTL组和TD组皮肤微生物菌群之间功能差异主要富集在新陈代谢相关通路。总之,该项研究揭示不同健康状态下棘胸蛙皮肤微生物菌群的组成和变化,突出共生微生物菌群在两栖动物健康养殖和疾病预防中的潜在应用。
The microbiota plays a critical role in amphibian health,however,the exact impact of disease on the host microbiota assembly remains unclear,and microbiota-based health assessment and disease prediction is a promising yet poorly established field.Chinese spiny frog(Quasipaa spinosa)is an important“famous,high-quality and new”cultured amphibian in China,but diseases have become a major bottleneck restricting the development of breeding industry,especially during the initial stages of complete metamorphosis.Disruption of homeostasis or failure to reconstruct the skin microbiota leads to a variety of diseases.In this study,Illumina high-throughput sequencing technology was utilized to systemically explore potential associations between host health status(healthy spiny frogs(CTL),dysplastic disease(DD),and torticollis disease(TD))and the composition,diversity and function of the skin microbiota.A total of 2997 amplicon sequence variants(ASVs)were obtained by sequencing,quality control and clustering,annotating 32 phyla,76 orders,167 orders,259 families and 402 genera.Proteobacteria,Firmicutes and Bacteroidota were the dominant phyla(>1%)shared by all groups,but their abundance was significantly modified by health status of the host.At the genus level,50 sensitive taxa were screened with 23(e.g.,Lactobacillus,Devosia,Shewanella and Luteolibacter)exhibiting high relative abundance in the CTL group,indicating the health status of spiny frogs.19 taxa(e.g.,Empedobacter,Bacillus and Brevinema)showed high relative abundance in the DD group,indicating dysplasia in spiny frogs,and 8 taxa(e.g.,Elizabethkingia,Citrobacter and Acinetobacter)had high relative abundance in the TD group,indicating torticollis.Alpha diversity analyses indicated that the TD group had significantly lower diversity and evenness compared to the CTL and DD groups,and the microbiota composition also exhibited distinct separation.Functional predictions indicated significant differences between the CTL and diseased groups,which were mainly enriched in metabolism-relevant pathways.In summary,this study depicts the composition and changes in the skin microbiota of spiny frogs under different health conditions,highlighting the potential application of symbiotic microbiota modification in healthy amphibian culture and disease prevention.
作者
徐杨
江昀
侯金亮
李娟
冯兴浪
刘新华
李德亮
向建国
李军华
XU Yang;JIANG Yun;HOU Jin-Liang;LI Juan;FENG Xing-Lang;LIU Xin-Hua;LI De-Liang;XIANG Jian-Guo;LI Jun-Hua(College of Fisheries,Hunan Agricultural University,Changsha 410128,China)
出处
《水生生物学报》
CAS
CSCD
北大核心
2024年第5期839-848,共10页
Acta Hydrobiologica Sinica
基金
国家自然科学基金(32173021)
湖南省自然科学基金(2021JJ30326)
湖南省现代农业科研体系(2019-105)资助。
关键词
变态末期
发育不良
歪头病
皮肤微生物菌群
棘胸蛙
Complete metamorphosis
Dysplasia
Torticollis
Skin microbiota
Quasipaa spinosa