微小RNA-185水平对结直肠癌术后无进展生存有评估价值

Expression of miRNA-185:a potential marker of progression-free survival after colorectal cancer

目的:探讨微小RNA(miRNA)-185表达与结直肠癌临床病理特点的关联及其对预后的评估价值。方法:回顾性分析手术治疗的130例结直肠癌患者的临床资料,使用荧光定量聚合酶链反应(PCR)技术检测癌组织标本和癌旁正常组织中的miRNA-185表达水平,分析不同临床病理资料特征下miRNA-185表达水平差异。通过受试者工作特征(ROC)曲线和Cox多因素回归分析miRNA-185对预后的评估价值。结果:结直肠癌组织中miRNA-185水平低于癌旁正常组织(0.176±0.085 vs 0.364±0.113,P<0.05),不同性别、年龄、分化程度、肿瘤位置和肿瘤大小特征下miRNA-185表达水平无统计学差异(P均>0.05),miRNA-185在Ⅲ~Ⅳ期结直肠癌、淋巴结转移肿瘤组织中表达水平更低(P均<0.05)。对评估术后肿瘤是否进展进行ROC曲线分析显示,miRNA-185的曲线下面积为0.816,95%CI:0.716~0.923,灵敏度为70.21%,特异性为83.23%,多因素Cox结果显示,TNM分期为Ⅲ~Ⅳ期(HR=2.417,95%CI:1.362~4.289),miRNA-185低表达(HR=3.121,95%CI:1.603~6.077)是预后的危险因素。结论:miRNA-185在结直肠癌组织中表达水平下调,并能作为潜在的评估结直肠癌术后无进展生存的标志物。

Objective:To evaluate the expression of miRNA-185 in different clinicopathological characteristics of colorectal cancer and its value as a prognostic marker.Methods:Totally,130 patients with colorectal cancer were retrospectively selected.The expression level of miRNA-185 was detected in cancer tissue specimens and normal tissues adjacent to cancer by fluorescence quantitative PCR.The miRNA-185 expression in different clinicopathological data was compared.Multivariate Cox regression and ROC curve were used to evaluate the prognostic value of miRNA-185.Results:The level of miRNA-185 in colorectal cancer tissues was significantly lower than that in normal tissues adjacent to cancer(0.176±0.085 vs.0.349±0.114,P<0.05).The expression level of miRNA-185 was not significantly correlated with gender,age,degree of differentiation,tumor location and size(P>0.05),but the expression was lower in stage Ⅲ-Ⅳ colorectal cancer and lymph node metastasis tumor tissues(P<0.05).The ROC curve analysis of postoperative tumor progression showed that the area under the curve of miRNA185 was 0.816(95CI%:0.716-0.923),sensitivity was 70.21%,and specificity was 83.23%.Multivariate Cox analysis showed TNM stage Ⅲ-Ⅳ(HR = 2.417,95%CI:1.362-4.289),miRNA-185 low expression(HR=3.121,95%CI:1.603-6.077) were prognostic risk factors.Conclusion:The expression of miRNA-185 is down-regulated in colorectal cancer tissues and can be used as a potential marker of progression-free survival after colorectal cancer surgery.