Ets-1和survivin在宫颈鳞状细胞癌中的表达研究

Expression of Ets-1 and Survivin in Cervical Squamous Cell Carcinoma

目的 探讨宫颈鳞状细胞癌(宫颈鳞癌)Ets-1和survivin表达与患者临床病理指标的关系。方法应用免疫组化EliVision^(TM) plus二步法检测72例宫颈鳞癌手术标本及30例癌旁宫颈组织Ets-1和survivin表达,分析其表达与患者年龄、癌灶大小、浸润深度、分化等级、TNM分期、术前淋巴转移、术后5年内复发转移7项指标的关系。结果 Ets-1阳性染色可见于细胞核及细胞质,survivin阳性染色多见于细胞质,少见于细胞核。癌组织Ets-1和survivin阳性率(分别为63.9%,72.2%)均显著高于癌旁组织(分别为13.3%,3.3%)(P<0.05)。患者年龄、癌灶大小均与Ets-1和Survivin表达阳性率无关(P>0.05);浸润深度、分化等级、TNM分期、术前淋巴转移、术后5年内复发转移5项指标均与Ets-1和survivin表达阳性率相关(P<0.05)。72例患者Ets-1和survivin共阳率为58.3%,共阴率为22.2%,两者表达呈正相关(r=0.49,P<0.05)。结论 Ets-1和survivin与宫颈鳞癌的发生发展密切相关,两者高水平表达预示癌组织侵袭能力强、分化等级低、病情发展快、淋巴转移早、患者预后差。

Objective To explore the expression of Ets-1 and survivin in cervical squamous cell carcinoma tissue and the relationship with clinicopathologic parameters.Methods Immunohistochemical EliVision^(TM) plus two-step method was used to detect the expression of Ets-1 and survivin in 72 pieces of cervical squamous cell carcinoma tissues and 30 pieces of adjacent carcinoma tissues.The relationship between the expression of Ets-1 and survivin and the clinicopathologic parameters including patient age,tumor size,depth of invasion,differentiated degree,TNM staging,lymphatic metastasis before operation,recurrence and metastasis in 5 years after surgery were studied.Results Ets-1 positive staining was mainly localized in the cell nucleus and cytoplasm.Survivin positive staining was mainly localized in cytoplasm,and lesser in the cell nucleus.The differences of positive rates of Ets-1 and survivin between cervical squamous cell carcinoma tissues(63.9%,72.2% respectively)and adjacent carcinoma tissues(13.3%,3.3% respectively)were statistically significant(P<0.05).The expression of Ets-1 and survivin was not statistically significant in the groups of different ages and tumor size(P>0.05).The Ets-1 and survivin expression was significantly correlated with the depth of invasion,differentiated degree,TNM staging,lymphatic metastasis before operation,recurrence and metastasis in 5 years after surgery(P<0.05).The rate of co-positive expression was 58.3%,and the rate of co-negative expression was 22.2%.There was a positive correlation between the expression of Ets-1 and survivin in cervical squamous cell carcinoma tissues(r=0.49,P<0.05).Conclusion Both expression of Ets-1 and survivin significantly take part in the development of cervical squamous cell carcinoma.The high expression of Ets-1 and survivin may hint strong invasion,poor differentiation,rapid progress,early lymphatic metastasis and poor prognosis.