激光散斑衬比成像技术在慢性脑缺血动物模型中的应用研究进展

Advances in the application of laser speckle contrast imaging in animal models of chronic cerebral hypoperfusion

激光散斑衬比成像技术采用可视化、可量化的方法实时动态测量组织或器官的微循环血流量。因其活体检测、实时成像及操作简单等优点,已在微循环检测的多个领域中应用。慢性脑缺血动物模型主要通过减少双侧颈总动脉向大脑输送血流量的方法造模,可造成脑供血长期不足。激光散斑衬比成像技术可通过对慢性脑缺血动物模型皮层脑血流量、侧支循环开放、神经血管耦合反应等进行观察分析,从而判断动物模型造模是否成功及干预方法对其的治疗效果。本文就激光散斑衬比成像技术在慢性脑缺血动物模型中的应用及其优缺点进行总结,以期为临床治疗和科研提供新思路。

A visual and quantitative method of dynamically measuring microcirculatory blood flow in a tissue or organ in real time was developed using laser speckle contrast imaging technology.It has been used in numerous microcirculation detection domains because of its benefits of in vivo detection,real⁃time imaging,and ease of operation.Most animal models of chronic cerebral hypoperfusion involve decreasing blood flow to the brain from bilateral common carotid arteries,which can lead to a protracted deficit in cerebral blood supply.By monitoring and analyzing cortical cerebral blood flow,collateral circulation opening,and neurovascular coupling responses,laser speckle contrast imaging technology assesses the efficacy of modeling and the therapeutic effect of interventions in animal models of chronic cerebral hypoperfusion.This article summarizes the benefits and drawbacks of laser speckle contrast imaging in an animal model of chronic cerebral hypoperfusion to promote novel approaches to clinical care and academic study.