超声改性甲壳素吸附水产品加工副产物中脂溶性维生素的作用效果研究

Adsorption effects of ultrasonic modified chitin on fat-soluble vitamins extracted from the by-products of aquatic product processing

目的探讨超声改性甲壳素(ultrasonic modified chitin,UM-C-chitin)对水产品加工副产物中脂溶性维生素的吸附效果。方法商品甲壳素(commercial chitin,C-chitin)经超声法改性,采用扫描电镜观察甲壳素改性前后微观形貌,傅里叶变换红外光谱和X射线衍射分析甲壳素改性前后的官能团和衍射峰变化,检测UM-C-chitin对水产品加工副产物中脂溶性维生素(维生素A、维生素D和维生素E)静态吸附效果。结果C-chitin经超声处理后微观结构由致密向疏松转变,并伴有无规则孔道及小孔形成,UM-C-chitin的酰胺I基团含有与α-型甲壳素相关的1665cm^(-1)特征峰,但是1624cm^(-1)处特征峰强度减弱接近消失,X射线衍射分析中UM-C-chitin在9.18°、12.68°、19.12°、23.04°和26.16°处有α-型甲壳素特征衍射峰,但各衍射峰强度较C-chitin的出现不同程度降低。与C-chitin相比较,UM-C-chitin对水产品加工副产物中维生素E的吸附效果较好(P<0.05),吸附量达到6.10μg/mg,较C-chitin的提高了84.8%。结论超声改性会降低C-chitin的结晶度,形成的无规则孔道及小孔结构有助于提高维生素E的负载量。本研究为快速富集水产品加工副产物中脂溶性维生素,特别是维生素E提供了技术依据。

Objective To investigate the adsorption effects of ultrasonic modified chitin(UM-C-chitin)on fat-soluble vitamins derived from the by-products of aquatic product processing.Methods Ultrasonic treatment was used to modify commercial chitin(C-chitin).The microstructure of chitin before and after ultrasonic modification was observed using scanning electron microscopy,as well as Fourier transform infrared spectroscopy and X-ray diffraction were conducted to analyze the changes in functional groups and diffraction peaks.Furthermore,the static adsorption effect of UM-C chitin on fat-soluble vitamins(vitamin A,vitamin D,and vitamin E)derived from the by-products of aquatic product processing was determined.Results After ultrasonic treatment,the microstructure of C-chitin transformed from dense to loosen,accompanied by the formation of irregular and small pores.The amide I group of UM-C-chitin had a characteristic peak at 1665 cm^(-1)related to a-chitin,however,the intensity of the characteristic peak at 1624 cm^(-1)weakened and almost disappeared.In X-ray diffraction analysis,the diffraction peaks of UM-C chitin at 9.18°,12.68,19.12°,23.04°,and 26.16°indicated itsα-chitin property.However,compared to C-chitin,the intensities of these diffraction peaks were all decreased to some degree in UM-C chitin.UM-C chitin had significantly higher adsorption capacity for vitamin E derived from the by-products of aquatic product processing than C-chitin(P<0.05),with 6.10μg/mg of adsorption content,increased by 84.8%compared to C-chitin.Conclusion Ultrasonic modification can reduce the crystallinity of C-chitin.The irregular and small pore structures formed help to increase the loading capacity of vitamin E.The results will provide a technical basis for the rapid enrichment of fat-soluble vitamins,especially vitamin E,from the by-products of aquatic product processing.