广东地区46株食源性金黄色葡萄球菌耐药性、生物被膜形成能力及IcaAB基因的特征分析

Characteristion of antibiotic resistance,biofilm formation ability and IcaAB genes in 46 strains of foodborne Staphylococcus aureus in Guangdong Province

目的分析广东地区食源性金黄色葡萄球菌耐药现状、生物被膜形成能力及生物被膜相关基因IcaAB的特征。方法对46株食源性金黄色葡萄球菌采用微量肉汤稀释法进行药敏实验,采用结晶紫法检测生物被膜生成能力,运用聚合酶链式反应(polymerase chain reaction,PCR)检测生物被膜相关基因。结果97.83%的菌株对至少1种抗生素耐药,30.43%为多重耐药菌株,对青霉素(97.83%)、氨苄西林(97.83%)耐药性最高;65.22%菌株能够形成生物被膜,其中强、中和弱粘附生物被膜能力的菌株分别占17.39%、30.43%和17.39%,且产生物被膜能力强的菌株对特定抗生素(头孢西丁、环丙沙星)的耐药性更高(P<0.05);PCR结果显示,icaA、icaB的检出率分别为56.67%、36.67%,基因携带率与产膜能力无显著关联(P>0.05)。结论广东地区食源性金黄色葡萄球菌普遍表现出耐药性和生物被膜形成能力,对公共卫生带来挑战,需采用措施以确保公共安全。

Objective To analyze the current status of drug resistance,biofilm formation ability,and the presence of the biofilm-related gene IcaAB in foodborne Staphylococcus aureus(S.aureus)in Guangdong Province.Methods Forty-six strains of S.aureus isolated from food were tested by microdilution method,crystal violet was used to detect the generating capacity of biofilm,and polymerase chain reaction(PCR)was used to detect biofilm related genes.Results The results showed that 97.83%of the strains were resistant to at least one antibiotic,among which 30.43%were multi-drug resistant strains.The highest resistance was found to penicillin(97.83%)and ampicillin(97.83%).The results showed that 65.22%of the strains could form biofilms,among which the strains with strong,medium and weak biofilm formation abilities accounted for 17.39%,30.43%and 17.39%respectively.And strains with a strong biofilm-forming ability more resistant to specific antibiotics(cefoxitin,ciprofloxacin)(P<0.05).PCR results showed that the detection rates of icaA and icaB were 56.67%and 36.67%respectively,with no statistical significance in gene carriage rates among strains with different biofilm formation abilities.ConclusioSn.aureus in food in the Guangdong region of China commonly demonstrates antibiotic resistance and biofilm-forming ability,posing a challenge to public health.Measures need to be taken to ensure public safety.