黑水虻幼虫粉对罗氏沼虾生长、免疫和抗氧化性能的影响

Effects of black fly larvae powder on growth,immunity,and antioxidant performance of Macrobrachium rosenbergii juveniles

试验旨在探究饲料中不同比例的黑水虻幼虫粉替代鱼粉对罗氏沼虾(Macrobrachium rosenbergii)生长和免疫功能的影响。选取900尾健康、体重(1.22±0.02) g的罗氏沼虾幼虾,随机分为6组,每组3个重复,每个重复50尾虾,对照组(FM组)饲喂基础饲料,T10组、T20组、T30组、T40组、T50组分别使用黑水虻幼虫粉替代基础饲料中10%、20%、30%、40%和50%的鱼粉。试验期56 d。结果显示,FM组和T10组的饲料系数显著低于其他组(P<0.05),FM组蛋白质效率显著高于其他组(P<0.05)。FM组、T10组、T20组的血淋巴丙二醛(MDA)含量显著低于T40组、T50组(P<0.05),T10组的血淋巴溶菌酶(LZM)活性显著高于T30组、T40组、T50组(P<0.05),T30组、T40组肝胰脏碱性磷酸酶(AKP)及酸性磷酸酶(ACP)活性低于其他试验组。研究表明,使用黑水虻幼虫粉替代10%~20%的鱼粉饲喂罗氏沼虾是可行的。

The experiment aimed to explore the impact of replacing fish meal with black fly larvae powder at different ratios in the feed on the growth and immune function of Macrobrachium rosenbergii.Nine hundred healthy juvenile Macrobrachiumrosenbergii with an average weight of(1.22±0.02)g were randomly divided into six groups,with three replicates per group and 50 shrimp per replicate.The control group(FM group)received a basal diet,while the T10 group,T20 group,T30 group,T40 group,and T50 group had 10%,20%,30%,40%,and 50% of fish meal replaced by black fly larvae powder,respectively.The experimental period lasted for 56 days.The results showed that the feed conversion ratio of the FM group and T10 group was significantly lower than that of the other groups(P<0.05),and the protein efficiency of the FM group was significantly higher than that of the other groups(P<0.05).The blood lymphatic malondialdehyde(MDA)content of the FM group,T10 group and T20 group was significantly lower than that of the T40 group and T50 group(P<0.05).The blood lymphatic lysozyme(LZM)activity of the T10 group was significantly higher than that of the T30 group,T40 group,and T50 group(P<0.05).The liver and pancreas alkaline phosphatase(AKP)and acid phosphatase(ACP)activities of the T30 group and T40 group were lower than those of the other experimental groups.The study suggests that feeding Macrobrachiumrosenbergiiwith a diet where 10%to 20% of fish meal is replaced by black fly larvae powder is feasible.