经方黄精酒对银屑病样小鼠的作用研究

Effect of Jingfang Huangjing liquor on psoriasis-like mice

研究经方黄精酒对银屑病样小鼠的治疗作用。采用5%咪喹莫特(IMQ)乳膏构建银屑病样小鼠模型,考察空白对照组、模型组、甲氨蝶呤组、经方黄精酒低剂量组(5 mL/(kg·d))、中剂量组(10 mL/(kg·d))、高剂量组(20 mL/(kg·d))、黄酒组(20 mL/(kg·d))、水提液组(20 mL/(kg·d))对银屑病样小鼠病样皮损、炎症反应和氧化应激水平的影响。结果显示:与模型组相比,经方黄精酒低中高剂量组小鼠体质量下降及脾肿大表现均得到有效缓解;苏木精-伊红(HE)染色结果显示,经方黄精酒组小鼠表皮层厚度明显变薄,真皮层中炎症细胞浸润程度较轻,皮损区域有所改善,且剂量越高其改善效果越明显;经方黄精酒低、中、高剂量组IL-17A表达水平均极显著降低(P<0.01),丙二醛含量均极显著降低(P<0.01),过氧化氢酶(CAT)含量均显著提升(P<0.05);经方黄精酒高剂量组超氧化物歧化酶(SOD)活性极显著提升(P<0.01),而低、中剂量组活性未显著改变(P>0.05)。综合来看,经方黄精酒高剂量组(20 mL/(kg·d))对由咪喹莫特所致银屑病样小鼠症状具有显著改善作用。

The therapeutic effect of the traditional Chinese medicine Huangjing liquor on psoriasis-like mice was studied.A psoriasis-like mouse model was constructed with 5%imiquimod(IMQ)cream,the effect of blank control group,model group,methotrexate group,low-dose Huangjing liquor group(5 ml/(kg·d)),medium-dose group(10 ml/(kg·d)),high-dose group(20 ml/(kg·d)),Huangjing liquor group(20 ml/(kg·d)),and water extract group(20 ml/(kg·d))on the levels of diseased skin lesions,inflammatory response and oxidative stress in psoriatic mice was investigated.The results showed that compared to the model group,effective alleviation of decreased body weight and splenomegaly was observed in the low,medium,and high-dose Huangjing liquor groups.Hematoxylin-eosin(HE)staining results demonstrated that the epidermal layer thickness of mice in the Huangjing liquor group significantly decreased,the degree of inflammatory cell infiltration in the dermis was milder,and there was improvement in the affected skin area,with a more pronounced effect at higher doses.The expression levels of IL-17A were highly significantly reduced in the low,medium,and high-dose Huangjing liquor groups(P<0.01).The malonaldehyde content was highly significantly decreased(P<0.01),and the catalase(CAT)content was significantly increased(P<0.05)in all Huangjing liquor groups.The superoxide dismutase(SOD)activity was highly significantly increased(P<0.01)in the high-dose Huangjing liquor group,while there was no significant change in activity in the low and medium-dose groups(P>0.05).In conclusion,the high-dose Huangjing liquor group(20 ml/(kg·d))had a significant improvement effect on the symptoms of psoriasis-like mice induced by imiquimod.