高效液相色谱法测定人参保健酒中11种人参皂苷含量

Determination of 11 ginsenosides in ginseng health wine by HPLC

该研究建立了高效液相色谱法(HPLC)同时测定人参保健酒中11种人参皂苷(人参皂苷Rg1、Re、Rf、Ra2、Rb1、Rc、Ra1、Ro、Rb2、Rb3、Rd)的方法。采用Agilent SB C_(18)色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%磷酸为流动相梯度洗脱,流速1.0 mL/min,柱温28℃,人参皂苷的检测波长203 nm,进样量10μL的条件进行测定。结果表明,11种人参皂苷在各自浓度范围内线性关系良好(R^(2)≥0.9999),检出限在1.52~3.03 mg/L,定量限在4.59~9.18 mg/L,该方法精密度试验、稳定性试验、重复性试验结果相对标准偏差(RSD)均小于5%,平均加标回收率95.39%~101.96%,RSD为0.07%~2.46%。该方法操作简单,准确度高,重复性好,可用于人参保健酒中11种人参皂苷含量测定。

In this study,a high performance liquid chromatography(HPLC)method was established for the simultaneous determination of 11 ginsenosides(ginsenoside Rg1,Re,Rf,Ra2,Rb1,Rc,Ra1,Ro,Rb2,Rb3,Rd)in ginseng health wine.Using Agilent SB C_(18)column(4.6 mm×250 mm,5μm),with acetonitrile-0.1%phosphoric acid as mobile phase gradient elution,flow rate 1.0 ml/min,column temperature 28℃,the detection wavelength of ginsenoside was 203 nm,and the injection volume was 10μl.The results showed that 11 ginsenosides all had good linear relationship in their respective concentration range(R^(2)≥0.9999).The limits of detection were 1.52-3.03 mg/L,and the limits of quantitation were 4.59-9.18 mg/L.The relative standard deviation(RSD)of precision test,stability test and repetition test was less than 5%.The average recovery rate was 95.39%-101.96%,RSD was 0.07%-2.46%.The method had the advantages of simple operation,high accuracy and good repeatability,and could be used for the determination of 11 ginsenosides in ginseng health wine.