小花清风藤中三萜类成分的研究

Study on Triterpenoids of Sabia parviflora

目的:对小花清风藤Sabia parviflora Wall.ex Roxb.茎叶的化学成分进行研究。方法:采用硅胶柱、Sephadex LH-20葡聚糖凝胶、制备HPLC等色谱方法,对小花清风藤茎叶中的化学成分进行分离纯化,根据理化性质和波谱数据分析鉴定化合物结构。结果:从小花清风藤茎叶的醇提物中分离得到15个三萜类化合物,分别鉴定为:3β-羟基-Δ^(11,13(18))-齐墩果二烯(1)、3-氧-11α-羟基,Δ^(12)-齐墩果烯(2)、3β-羟基-Δ^(9(11),12)-齐墩果二烯(3)、3-氧-Δ^(11,13(18))-齐墩果二烯(4)、3α-羟基-齐墩果酸甲酯(5)、3-氧-Δ^(9(11),12)-齐墩果二烯(6)、3α,21β-二羟基,Δ^(12)-齐墩果烯(7)、2α,3α-二羟基-12-烯-28-乌苏酸(8)、马斯里酸(9)、积雪草酸(10)、2α-羟基乌苏酸(11)、23-羟基乌苏酸(12)、柴胡皂苷b_3(13)、柴胡皂苷c(14)、柴胡皂苷f(15)。结论:除化合物5以外,其他化合物均为首次从该植物中分离得到,化合物7~15为首次从该属植物中分离得到。

Objective:To study the chemical constituents of Sabia parviflora.Methods:The chemical constituents of Sabia parviflora were isolated and purified by silica geg,Sephadex LH-20,pre-HPLC and others.The structures were identified by physicochemical prop-erties and spectral data.Results:Fifteen compounds were isolated from ethanol extract of Sabia parviflora stems and leaves and identi-fied as 3β-hydroxy-olean-11,13(18)-diene(1),3-oxo,11α-hydroxy-olean-12-ene(2),3β-hydroxy-olean-9(11),12-diene(3),3-oxo-olean-11,13(18)-diene(4),methyl-3α-hydroxyl-olean-12-ene-28-oate(5),3-oxo-olean-9(11),12-diene(6),3α,21β-dihydroxy-olean-12-ene(7),2α,3α-dihydroxy-urs-12-en-28-oic acid(8),masilinic acid(9),asiatic acid(10),2α-hydroxyursolic acid(11),23-hydroxyursolic acid(12),saikosaponin b3(13),saikosaponin c(14),saikosaponin f(15),respectively.Conclusion:Except for com-pound 5,all the other compounds are isolated from Sabia parviflora for the first time,compounds 7 to 15 are isolated from Sabia genus for the first time.