莜麦类甜蛋白Permatin抑制木霉菌活性的机制研究

Mechanism on the inhibitory activity of Avena thaumatin-like proteins Permatin against Trichoderma

类甜蛋白(thaumatin-like proteins,TLPs)是一个高度复杂的蛋白质家族,与植物、动物和真菌的宿主防御和发育过程有关。TLPs已在多种植物中被发现,具有广谱抗真菌活性。近年来,植物来源的抗真菌蛋白在植物抗病原菌中具有广泛的应用前景,然而关于莜麦来源的抗真菌蛋白研究比较少。本研究从莜麦中分离纯化得到莜麦类甜蛋白Permatin,通过带毒平板法测定了Permatin蛋白对木霉菌的半抑制浓度;然后利用Delta Vision显微镜观察了Permatin蛋白对木霉菌孢子萌发及菌丝生长的影响;进一步结合碘化丙啶(propidium Iodine,PI)染色研究Permatin蛋白对木霉菌菌丝及孢子细胞膜通透性的影响。结果显示,Permatin蛋白对木霉菌的半抑制浓度为0.73μmol/L,可以显著抑制木霉菌孢子的萌发及菌丝的生长,并且能够破坏真菌菌丝及孢子细胞膜的通透性。本研究为莜麦Permatin蛋白在农业领域的应用提供了理论基础。

Thaumatin-like proteins(TLPs)are a highly complex protein family associated with host defense and development processes in plants,animals and fungi.TLPs have been found in a variety of plants with broad-spectrum anti-fungal activity.In recent years,the anti-fungal proteins derived from plants have a wide range of application prospects in plant resistance to pathogenic bacteria.However,there are relatively few studies on anti-fungal protein derived from Avena.In this study,the Permatin was isolated and purified from Avena.The half inhibitory concentration of Permatin against Trichoderma was determined by Radial Growth Assay.Then,the effect of Permatin on spore germination and mycelia growth of Trichoderma was observed by Delta Vision microscope.The effect of Permatin on the permeability of Trichoderma mycelia and spores cell membrane was further investigated by combining with Propidium Iodide Staining.The results showed that the half inhibitory concentration of Permatin on Trichoderma was O.73μmol/L,which could significantly inhibit the spores germination and mycelium growth of Trichoderma,and could also destroy the permeability of fungal mycelium and spore cell membranes.This study provides a theoretical basis for the application of Permatin from Avena in agriculture.