基于适配体的纳米金比色法检测中药材中黄曲霉毒素B_(1)

Colorimetric Determination of Aflatoxin B_(1) in Chinese Herbal Medicine based on Gold Nanoparticles and Aptamers

中药材贮存加工过程中易受真菌毒素污染,其中黄曲霉毒素B_(1)(AFB_(1))以其高毒性和致癌性备受关注。制备了金纳米粒子作为基质,采用核酸适配体作为特异性识别探针,构建了一种快速可视化检测中药材中AFB_(1)的方法。该方法检测AFB1的线性范围为2.5~100 ng/mL,检出限为1.87 ng/mL。应用该方法测定麦芽、决明子、三七中AFB_(1)的含量,加标回收率为89.1%~109.7%,相对标准偏差为3.0%~8.3%。结果表明本方法具有良好的选择性和准确性。

Chinese herbal medicine is susceptible to be contaminated by mycotoxins during the storage and processing.Among the mycotoxins,aflatoxin B1(AFB_(1))has attracted much attention due to its high toxicity and carcinogenicity.A rapid and visul detection method of AFB_(1) in Chinese herbal medicine was established.In this method,gold nanoparticles were employed as the matrix and nucleic acid aptamer was used as specific recognition probe.The detection limit of AFB1 was 1.87 ng/mL and the linear detection range was from 2.5 to 100 ng/mL.The established method was employed for the detection of AFB1 in malt,Semen Cassiae and Panax notoginseng.The spiked recovery tests of different samples revealed that the recovery rates were between 89.1% and 109.7%,and the relative standard deviation ranged from 3.0% to 8.3%.The results indicate that this method has good selectivity and accuracy.