蔗糖对参薯块茎形成及DamiR156/DaSPL的调控分析

Regulation and Analysis of Sucrose on Tuber Formation and DamiR156/DaSPL in Dioscorea alata

为了探究蔗糖对不同基因型参薯块茎形成的影响,以及对块茎发育过程中相关的DamiR156及其靶基因DaSPL的调控作用,本研究采用不同蔗糖浓度处理参薯组培苗Da40、Da70、Da207,结果表明高浓度的蔗糖能够促进Da40和Da70的块茎形成。当蔗糖浓度为90 g/L时,Da40的结薯时间提早了22 d,Da70的结薯时间提早了40 d。使用RT-qPCR技术测定不同蔗糖浓度处理下Da70的DamiR156及其靶基因DaSPL6相对表达量。结果表明,在第30天,DamiR156的表达量在蔗糖浓度为70 g/L时表达量最高。在第60天(即块茎形成初期),当蔗糖浓度为0 g/L时,DamiR156的表达量最高,DaSPL6表达量最低,在6个不同蔗糖浓度处理下,DaSPL6的表达量呈现先上升后下降的趋势,DaSPL6在70 g/L时表达量最高。研究结果表明外源蔗糖可促进参薯生长并诱导提前结薯,调节DamiR156/DaSPL6的表达,这为后续研究蔗糖调控参薯块茎形成的分子机制提供了一定的理论依据。

In order to explore the effects of sucrose on tuber formation of different genotypes of Dioscorea alata(great yam),as well as the regulatory effect of DamiR156 and its target gene DaSPL in the development of tubers,this study treated Da40,Da70,and Da207 of great yam with different sucrose concentrations,and the results showed that high concentrations of sucrose could promote tuber formation of Da40 and Da70.When the sucrose concentration was 90 g/L,the tuberculosis formation time of Da40 was 22 days earlier,and Da70 was 40 days earli-er.The relative expression of DamiR156 and its target gene DaSPL6 of Da70 under different sucrose concentration treatments was determined by RT-qPCR.The results showed that at 30 days,the expression of DamiR156 was high estat70g/L,andat60days(theearlystageoftuber formation),the expression of DamiR156 wasthe highest and DaS-PL6 was the lowest at a sucrose concentration of 0 g/L.Under the treatment of 6 different sucrose concentrations,the expression of DaSPL6 showed a trend of first rising and then decreasing,and DaSPL6 was the highest expres-sion at 70 g/L.The results showed that exogenous sucrose could promote the growth of great yam and induce early tuber formation while regulating the expression of DamiR156/DaSPL6 and providing a theoretical basis for the sub-sequent study of the molecular mechanism of sucrose regulating the tuber formation of great yam.