GC-MS/MS测定植物油中生育酚和生育三烯酚

Determination of tocopherols and tocotrienols in vegetable oils by GC-MS/MS

建立一种气相色谱-串联质谱法(GC-MS/MS)同时测定植物油中α-、β-、γ-、δ-生育酚和α-、β-、γ-、δ-生育三烯酚含量的方法。样品经异辛烷超声提取后,采用动态多反应监测离子模式分析。结果表明:8种维生素E异构体可实现基线分离,在1~500 ng/mL范围内,线性关系良好,相关系数均大于0.999,检出限和定量限分别为0.02~0.05 mg/kg和0.10 mg/kg,平均回收率为81.5%~109.5%,相对标准偏差为3.3%~7.2%。方法快速、简便、准确、灵敏度高,适用于植物油中生育酚和生育三烯酚含量的测定。

A method was established for the simultaneous determination of the content of α-β--,8-tocopherol and α-、β-、y-、α-tocotrienolin in vegetable oils by gas chromatography-tandem mass spectrometry(GC-MS/MS).The sample was extracted by isooctane,and analyzed by dynamic multi reaction monitoring ion mode.The results showed that 8 types of vitamin E could achieve baseline separation,with good linear relationships in the range of 1-500ng/mL,correlation coefficients greater than 0.999,detection limits and quantification limits of 0.02-0.05 mg/kg and 0.10mg/kg,respectively.The average recovery rate was 81.5%-109.5%,and the relative standard deviation was 3.3%-7.2%.The method is fast,simple,accurate,and highly sensitive,and is suitable for the determination of tocopherol and tocotrienol contents in vegetable oils.