镍钛泡沫合金对成骨细胞生物活性的影响

Effect of porous nitinol on bioactivity of preosteoblast cells

目的观察镍钛泡沫合金(PNT)在体外实验对细胞的生物安全性。方法选择PNT和镍钛致密合金(NiTi)材料(中南大学材料科学工程学院提供),以及纯钛(Ti)(宁波慈北医疗器械有限公司,中国);MC3T3-E1来源于中国科学院典型培养物保藏委员会细胞库。用PNT、NiTi、Ti 3种合金的浸提液,培养小鼠前成骨细胞MC3T3-E1。用CCK-8法检测细胞的增殖活性,碱性磷酸酶法测定细胞的分化能力,流式细胞术检测细胞早期凋亡情况。结果PNT组细胞增殖能力在第1天、第3天、第7天均明显小于NiTi组(P<0.001)和Ti组(P<0.001)。PNT组细胞分化能力在第1天、第3天、第7天均明显小于NiTi组(P<0.001)和Ti组(P<0.001)。PNT组细胞的凋亡细胞百分比在第1天、第3天、第7天均明显高于NiTi组细胞(P=0.001)和Ti组细胞(P<0.001)。结论PNT对细胞的增殖和分化的影响明显大于NiTi组和Ti组,更明显地抑制了细胞的增殖与分化能力,更容易导致细胞早期凋亡。

Objective To explore the biological safety effect of porous nitinol(PNT)on preosteoblast cells in vitro.Methods PNT and dense nitinol(NiTi)materials were provided by School of Materials Science and Engineering,Central South University.Ti was purchased from Ningbo Cibei Medical Instrument Co.LTD.MC3T3-E1 was derived from Typical Culture Preservation Committee Cell Bank of Chinese Academy of Sciences.Leaching liquor of PNT,NiTi and titanium(Ti)without surface treatment was used to culture preosteoblast MC3T3-E1 cells.The proliferation activity of the cells was detected by CCK-8 method,and alkaline phosphatase activity was detected to observe cell differentiative capacity,and mitochondrial membrane potential was assayed to observe early cell apoptosis.Results The cell proliferation capacity of PNT group was significantly lower than that of NiTi group(P<0.001)and Ti group(P<0.001)on 1st day,3rd day and 7th day.The cell differentiation ability of PNT group was significantly lower than that of NiTi group(P<0.001)and Ti group(P<0.001)on 1st day,3rd day and 7th day.Conclusion It is demonstrated that effect of PNT on cell proliferation and differentiation is significantly greater than that of NiTi and Ti groups,and it more obviously inhibits the proliferation and differentiation of cells,and is more likely to make early apoptosis.