环介导等温扩增技术在病原微生物临床检测中的应用

Application of loop-mediated isothermal amplification technique in pathogenic microorganisms clinical detection

病原微生物是威胁人类健康的重要因素之一,快速、准确的检测方法对于感染性疾病的诊断具有重要意义。环介导等温扩增(LAMP)是一种恒温扩增方法,具有反应时间短、操作简便、灵敏度高、特异度高、检测成本低等优点,因此被广泛应用于感染性疾病的快速诊断。基于这种情况,该文主要阐述了LAMP的原理、特点及其在临床常见病原微生物检测方面的应用与研究进展。LAMP技术现在已经被应用于病原微生物的检测,且具有较高的灵敏度及特异度,但该技术仍有易产生假阳性、引物设计复杂等不足之处。该文综述了近年来LAMP技术在病原微生物感染中的应用与进展,并对其未来的发展前景进行了展望,以期在资源有限的环境中为病原微生物感染的快速诊断提供合理的研究方向。

Pathogenic microorganisms are one of the important factors threatening human health.Rapid and accurate detection methods are of great significance for the diagnosis of infectious diseases.Loop-mediated isothermal amplification(LAMP)is one of the isothermal amplification methods,which has the advantages of short reaction time,simple operation,high sensitivity,high specificity and low cost,so it is widely used in the rapid diagnosis of infectious diseases.Based on this situation,this paper mainly elaborates the principle and characteristics of LAMP and its application and research progress in the detection of common pathogenic microorganisms in clinic.LAMP technology has been applied to the detection of pathogenic microorganisms with high sensitivity and specificity,but this technology is still prone to produce false positive,complex primer design and other shortcomings.This paper reviews the application and progress of LAMP technology in pathogenic microbial infection in recent years,and outlooks its future development prospect in order to provide a reasonable research direction for the rapid diagnosis of pathogenic microbial infection in the environment with limited resources.