活血止痛汤联合脂肪填充对大鼠胫骨骨骺损伤的影响

Effect of Huoxue Zhitong Decoction Combined with Fat Filling on the Injury of Fibial Epiphyseal in Fats

目的:探讨活血止痛汤联合脂肪填充对大鼠胫骨远端Ⅲ型和Ⅳ型骨骺损伤的作用。方法:36只大鼠随机分为对照组、模型组、脂肪组和脂肪中药组,采用阿利新蓝、免疫荧光染色观察损伤模型的建立及骨骺损伤愈合过程、骨桥形成过程及血管内皮生长因子(VEGF)、骨形态发生蛋白2(BMP2)和白细胞介素1β(IL-1β)的表达情况。结果:阿利新蓝染色结果显示:相较于对照组,模型组骨骺损伤明显并形成骨桥,然而加入脂肪和活血止痛汤干预后,骨骺损伤明显改善,骨桥消失,脂肪中药组效果更为明显。免疫荧光结果表明:对比对照组,模型组VEGF、BMP2和IL-1β的表达均有明显增加;而相较于模型组,脂肪组和脂肪中药组的VEGF、BMP2和IL-1β的表达均有所降低,并且脂肪中药组的VEGF、BMP2和IL-1β的表达下降更为明显。结论:活血止痛汤联合脂肪填充治疗大鼠胫骨远端Ⅲ型和Ⅳ型骨骺损伤疗效明显好于单纯脂肪干预。

Objective:To investigate the effect of Huoxue Zhitong decoction combined with fat filling on the type Ⅲ and Ⅳ epiphyseal injury of distal tibia in rats.Methods:36 rats were randomly divided into control group,model group,adipose group and adipose Chinese medicine group.The establishment of injury model,the healing process of epiphyseal injury,bone bridge formation and the expression of vascular endothelial growth factor(VEGF),bone morphogenetic protein-2(BMP2) and interleukin-1β(IL-1β) were observed by alcin blue staining and immunofluorescence staining.Results:Compared with the control group,alcin blue staining showed that the epiphyseal injury was obvious in the model group,and bone bridge was formed.However,after the intervention of adipose and Huoxue Zhitong decoction,the epiphyseal injury was obviously improved and bone bridge disappeared,and the effect of adipose Chinese medicine group was more obvious.Immunofluorescence results indicated that compared with the control group,the expressions of VEGF,BMP2 and IL-1β were significantly increased in the model group,while the expressions of VEGF,BMP2 and IL-1β were decreased in the fat group and the fat Chinese medicine group,and the expressions of VEGF,BMP2 and IL-1β were more significantly decreased in the fat Chinese medicine group.Conclusion:The therapeutic efficacy of Huoxue Zhitong decoction combined with fat filling on the type Ⅲ and Ⅳ epiphyseal injury of distal tibia in rats is significantly better than simple fat intervention.