芩术仙灵散对夏季公兔抗氧化能力、生殖激素水平和生殖能力的影响

Effects of Qinzhu Xianling Powder on antioxidant capacity,reproductive hormone level and reproductive capacity of male rabbits in summer

为了探究芩术仙灵散对夏季公兔抗氧化能力、生殖激素水平和生殖能力的影响,将18只健康、性成熟新西兰公兔随机分成3组,分别为常温对照组(全价饲料),高温高湿组(全价饲料)和给药组(全价饲料+芩术仙灵散2.5 g/d)。常温对照组饲养在温度25℃、相对湿度60%的动物房中,其他组饲养在夏季自然温度与光照的动物房中。记录试验兔日采食量和爬跨率,通过生化分析仪检测抗氧化能力,显微镜观察精子形态,放射免疫法检测生殖激素水平。结果:与常温对照组相比,高温高湿组公兔日采食量、爬跨率、抗氧化能力、精液品质和激素水平均显著降低(P<0.05),射精反应时间显著延长(P<0.05)。与高温高湿组比较,给药组公兔日采食量、爬跨率、抗氧化能力、激素水平均显著升高(P<0.05);射精反应时间缩短了3.67 s(P<0.05);精液品质改善明显,精子畸形率降低了1.72%(P<0.05),精子活率升高了8%(P<0.05)。本研究表明,公兔在夏季产生了热应激,日粮中添加芩术仙灵散可提高夏季公兔抗氧化能力、生殖激素水平和生殖能力。

In order to determine the effects of Qinzhu Xianling Powder on antioxidant capacity,reproductive hormone level and reproductive capacity of male rabbits in summer,18 healthy sexually mature male New Zealand rabbits were selected and randomly divided into three groups:the normal temperature control group(fed with basic diet),the high temperature and high humidity group(with basic diet)and the medication administration group(with basic diet plus 2.5 g/d Qinzhu Xianling Powder).The normal temperature control group was kept in an animal house at a temperature of 25℃and under a relative humidity of 60%,while the other groups were kept in a house at natural tem⁃perature and under natural light in summer.Then,daily feed intake and mounting rate of the rabbits were recorded,their antioxidant capacity was detected by biochemical analyzer,their sperm morphology was observed by microscope,and their reproductive hormone levels were de⁃tected by radioimmunoassay.The results showed that,compared with the normal temperature control group,the daily feed intake,mounting rate,antioxidant capacity,semen quality and hormone level of the male rabbits in the high temperature and high humidity group were signifi⁃cantly decreased(P<0.05),and their ejaculatory reaction time was significantly extended(P<0.05).Compared with the high temperature and high humidity group,the daily feed intake,mounting rate,antioxidant capacity and hormone level of the male rabbits in the medication administration group were significantly increased(P<0.05),their ejaculatory reaction time was shortened by 3.67 s(P<0.05);their sperm quality was improved significantly,their abnormal sperm rate was decreased by 1.72%(P<0.05),and their sperm motility rate was in⁃creased by 8%(P<0.05).In conclusion,Qinzhu Xianling Powder enhanced the antioxidant capacity,reproductive hormone level and repro⁃ductive ability of male rabbits in summer.