lncRNA-UCA1、miR-145-5p在妊娠期糖尿病孕妇胎盘及脐血中的表达及意义

Expression and significance of lncRNA-UCA1 and miR-145-5p in placenta and cord blood of pregnant women with gestational diabetes mellitus

目的探讨长链非编码RNA(lncRNA)尿路上皮癌胚抗原1(lncRNA-UCA1)、微小RNA-145-5p(miR-145-5p)在妊娠期糖尿病(GDM)孕妇胎盘组织、脐血中的表达水平及二者与胰岛素抵抗(IR)、新生儿体重的关系。方法选取2019年3月至2021年7月该院收治的86例由口服葡萄糖耐量试验(OGTT)确定为GDM的孕妇作为GDM组,另选取同期86例经OGTT判定为正常糖耐量(NGT)的孕妇为对照组。比较对照组和GDM组胎盘组织、脐血中lncRNA-UCA1、miR-145-5p表达水平及IR相关指标及新生儿体重,分析GDM孕妇胎盘组织、脐血lncRNA-UCA1、miR-145-5p表达水平与稳态模型胰岛素抵抗指数(HOMA-IR)、新生儿体重,以及胎盘组织、脐血lncRNA-UCA1表达水平与miR-145-5p的相关性。结果GDM组孕妇胎盘组织、脐血中lncRNA-UCA1表达水平低于对照组(P<0.05),miR-145-5p表达水平及空腹胰岛素(FINS)、空腹血糖(FBG)、HOMA-IR均高于对照组(P<0.05)。GDM孕妇胎盘组织、脐血lncRNA-UCA1表达水平与HOMA-IR、新生儿体重呈负相关(P<0.05),miR-145-5p表达水平与HOMA-IR、新生儿体重呈正相关(P<0.05),GDM孕妇胎盘组织、脐血lncRNA-UCA1表达水平与miR-145-5p呈负相关(P<0.05)。结论GDM孕妇胎盘、脐血中lncRNA-UCA1表达水平较低,miR-145-5p表达水平较高,二者均与IR、新生儿体重密切相关。

Objective To investigate the expression levels of long non-coding RNA(lncRNA)urothelial carcinoma-associated antigen 1(lncRNA-UCA1)and microRNA-145-5p(miR-145-5p)in placental tissue of pregnant women with gestational diabetes mellitus(GDM),and their relationship with insulin resistance(IR)and neonatal weight.Methods From March 2019 to July 2021,86 pregnant women with GDM in this hospital identified by oral glucose tolerance test(OGTT)were selected as the GDM group.In addition,86 pregnant women with normal glucose tolerance(NGT)determined by OGTT during the same period were taken as the control group.The levels of lncRNA-UCA1 and miR-145-5p in placental tissue and umbilical cord blood,IR-related indexes and neonatal body weight were compared between the control group and the GDM group.The correlation between the expression levels of lncRNA-UCA1 and miR-145-5p in placental tissue and cord blood of pregnant women with GDM and homeostasis model insulin resistance inde x(HOMA-IR),neonatal weight,and lncRNA-UCA1 expression levels in placental tissue and cord blood and miR-145-5p were analyzed.Results The expression level of lncRNA-UCA1 in placenta tissue and cord blood of pregnant women in GDM group was lower than those in control group(P<0.05),while the expression level of miR-145-5p,fasting insulin(FINS),fasting blood glucose(FBG),and HOMA-IR were higher than those in control group(P<0.05).The expression level of lncRNA-UCA1 in placenta tissue and cord blood of pregnant women with GDM were negatively correlated with HOMA-IR and neonatal weight(P<0.05),and the expression level of miR-145-5p was positively correlated with HOMA-IR and neonatal weight(P<0.05).The expression level of lncRNA-UCA1 in placenta tissue and cord blood of pregnant women with GDM was negatively correlated with miR-145-5p(P<0.05).Conclusion The expression level of lncRNA-UCA1 in placenta and cord blood of pregnant women with GDM are low,and the expression level of miR-145-5p are high,both of which are closely related to IR and neonatal weight.