血清miR-122-5p、miR-618、miR-202联合检测对原发性肝癌的诊断价值分析

Diagnostic value of serum miR-122-5p,miR-618 and miR-202 combined detection in primary liver cancer

目的分析血清微小核糖核苷酸-122-5p(miR-122-5p)、微小核糖核苷酸-618(miR-618)、微小核糖核苷酸-202(miR-202)联合检测对原发性肝癌的诊断价值。方法选取应急总医院2022年1月~2023年1月收治的原发性肝癌患者118例纳入原发性肝癌组,另择同期120例良性肝脏疾病患者纳入良性组,120例健康体检者为健康对照组,回顾性收集临床资料。比较3组研究对象血清miR-122-5p、miR-618、miR-202水平,通过Pearson相关性分析分析血清miR-122-5p、miR-618、miR-202水平之间的相关性;以原发性肝癌组作为阳性组,绘制受试者工作特征(ROC)曲线分析miR-122-5p、miR-618、miR-202单独及联合检测对原发性肝癌的诊断价值。结果原发性肝癌组血清miR-122-5p、miR-618、miR-202水平低于良性组和健康对照组,良性组低于健康对照组,差异有统计学意义(F=193.644、88.486、105.045,P<0.05)。年龄≥60岁、TNM分期Ⅲ~Ⅳ期、乙型肝炎病毒(HBV)感染、肿瘤数目多发的原发性肝癌患者血清miR-122-5p、miR-618、miR-202水平更低,差异有统计学意义(P<0.05)。Pearson相关性分析结果显示,原发性肝癌患者血清miR-122-5p、miR-618、miR-202间表达水平均呈正相关(P<0.05)。ROC曲线结果显示,miR-122-5p、miR-618、miR-202单独及联合检测对原发性肝癌的预测价值分析曲线下面积(AUC)分别为0.839、0.814、0.807、0.932,灵敏度分别为85.59%、84.75%、79.66%、88.98%,特异度分别为75.00%、68.75%、70.00%、85.42%,联合检测均为最高。结论随着原发性肝癌发生及病情进展患者血清miR-122-5p、miR-618、miR-202表达水平降低,联合检测对原发性肝癌的诊断价值较高。

Objective To analyze the diagnostic value of serum microrribonucleotide-122-5p(miR-122-5p),microrribonucleotide-618(miR-618)and microrribonucleotide-202(miR-202)combined detection in primary liver cancer.Methods A total of 118 patients with primary liver cancer admitted to our hospital from January 2022 to January 2023 were included in the primary liver cancer group,and 120 patients with benign liver disease were included in the benign group during the other period,with 120 healthy physical examination subjects were included in the healthy control group.Clinical data of all the 3 groups were retrospectively collected.The serum levels of miR-122-5p,miR-618,and miR-202 of liver cancer patients in the three groups with different pathological characteristics were analyzed.The correlation between serum levels of miR-122-5p,miR-618,and miR-202 was analyzed by Pearson correlation analysis.The receiver operating characteristic(ROC)curve was drawn to analyze the diagnostic value of miR-122-5p,miR-618,and miR-202 alone and combined in primary liver cancer.Results The serum levels of miR-122-5p,miR-618,and miR-202 in the primary liver cancer group were lower than those in the benign group and the healthy control group,and the benign group was lower than that in the healthy control group(F=193.644,88.486,105.045,P<0.05).Serum levels of miR-122-5p,miR-618,and miR-202 were lower in patients with primary liver cancer aged≥60 years,TNM stageⅢ~Ⅳ,hepatitis B virus(HBV)infection and multiple tumors(P<0.05).The result of the Pesrson correlation analysis showed that the expression levels of miR-122-5p,miR-618 and miR-202 in serum were positively correlated(P<0.05).Primary liver cancer was included in the positive group,benign group and healthy subjects in the same period were included in the negative group.ROC curve analysis result showed that the predictive value of miR-122-5p,miR-618,and miR-202 alone and combined detection on primary liver cancer,the area under the curve(AUC)were 0.839,0.814,0.807,and 0.932,the sensitivity was 85.59%,84.75%,79.66%,and 88.98%,and the specificity was 75.00%,68.75%,70.00%,and 85.42%respectively.The combined detection was the highest.Conclusion The expression levels of miR-122-5p,miR-618,and miR-202 in the serum of patients with primary liver cancer decrease with the occurrence and progression of the disease.Combined detection is of high diagnostic value for primary liver cancer.