甜玉米苗期耐铝性状QTL定位

QTL mapping of aluminum tolerance traits in sweet maize seedling stage

为挖掘甜玉米耐铝基因资源,以耐铝性状差异显著的甜玉米自交系‘T 49’(♀)和‘T 56’(♂)为亲本配制杂交组合‘T49×T56’,以208个F2单株作为遗传作图群体,构建玉米遗传连锁图谱,选取苗高系数、苗重系数、根长系数、根重系数和整株鲜重系数作为玉米耐铝性的评价指标。用复合区间作图法检测耐铝性状相关QTL。结果表明:在甜玉米10对染色体上获得10个SSR标记连锁群,全长1199.10 cM,平均间距7.83 cM。共检测到3个苗高系数QTL、4个苗重系数QTL、4个根长系数QTL、3个根重系数QTL、3个整株鲜重系数QTL,单个QTL可解释6.46%~25.37%的表型变异。在第9染色体上phi065-umc1271(bin 9.03-9.04),苗高系数、苗重系数、根长系数、根重系数、整株鲜重系数均检测到1个主效QTL,贡献率为13.1%~25.3%。结合GO、KEGG数据库,在phi065-umc1271内存在2个稳定的甜玉米非生物逆境胁迫耐受性相关的基因Zm00001d045472和Zm00001d046391。

In order to explore the aluminum tolerance gene resources in sweet maize,a hybrid combination‘T49×T56’was generated by taking two inbred lines‘T49’(♀)and‘T56’(♂),which have significant differences in aluminum tolerance characters,as the parents.A total of 208 F2 individual plants were used as genetic mapping population to construct the genetic linkage map of maize.Seedling height coefficient,seedling weight coefficient,root length coefficient,root weight coefficient and whole plant fresh weight coefficient were selected as the evaluation indexes.The aluminum tolerance of maize QTL related to aluminum resistance traits was detected by composite interval mapping.The results showed that:Ten SSR marker linkage groups were obtained on ten pairs of sweet maize chromosomes.The total length was 1199.10 cM,and the average distance was 7.83 cM.A total of 3 QTLS for seedling height,4 QTLS for seedling weight,4 QTLS for root length,3 QTLS for root weight,and 3 QTLS for whole plant fresh weight were detected.One single QTL could explain 6.46%to 25.37%of phenotypic variation.In the labeled intervals phi065—umc1271(bin 9.03-9.04)on chromosome 9,a major QTL was detected in the coefficient of seedling height,seedling weight,root length,root weight and whole plant fresh weight,and the contribution rate was 13.1%-25.3%.Combined with GO and KEGG databases,two stable genes Zm00001d045472 and Zm00001d046391 related to abiotic stress tolerance of sweet corn were found in phi065-umc1271.