毛竹SLAC家族基因鉴定及PheSLAC1功能分析

Identification of SLAC Gene Family in Phyllostachys edulis and Characterization of PheSLAC1

为了研究S型阴离子通道蛋白(slow type anion channel,SLAC)基因家族在毛竹(Phyllostachys edulis)响应干旱胁迫中的作用,利用生物信息方法以拟南芥和水稻中的SLAC/SLAH家族蛋白序列为模板对毛竹中SLAC家族成员进行鉴定,对其基因结构、进化关系和组织表达等进行分析,并对PheSLAC1的保守功能位点以及在干旱胁迫中的功能进行初步研究。结果表明:毛竹中包含15个SLAC家族基因,其中PheSLAC1.1(PH02Gene01933.t1)和Phe SLAC1.2(PH02Gene00592.t1)在叶片中表达量最高,基因编码区(coding sequence,CDS)序列长度分别为1 716和1 677 bp,分别编码572和559个氨基酸,且包含保守的关键功能位点。亚细胞定位结果表明,PheSLAC1.1和Phe SLAC1.2均定位于细胞质膜中。PheSLAC1.1和Phe SLAC1.2在拟南芥slac1-3突变体中的表达结果表明,其能够部分减轻突变体的干旱敏感表型,表明两者均能够通过调控气孔关闭在毛竹响应干旱胁迫中发挥重要作用。

To investigate the function of slow anion channel(SLAC)protein of Phyllostachys edulis in response to drought stress,genes encoding slow anion channel proteins in moso bamboo genome were identified by TBtools software based on SLAC/SLAH genes from Arabidopsis thaliana and Oryza sativa,respectively.Phylogenetic relationship,conserved region and motif,and gene expression pattern were investigated.Moreover,the function of PheSLAC1 in response to drought stress was verified by transgenic method.There were 15 members in SLAC family in bamboo,and the expression of PheSLAC1.1(PH02Gene01933.t1)and PheSLAC1.2(PH02Gene00592.t1)were the highest in leaves.The coding sequence(CDS)of PheSLAC1.1 and PheSLAC1.2 were 1716 and 1677 bp in length,encoding 572 and 559 amino acids,respectively.Subcellular localization exhibited that PheSLAC1.1 and PheSLAC1.2 were mainly located in cell membrane.Transgenic plants expressing PheSLAC1.1 and PheSLAC1.2 in slac1-3 mutants showed that PheSLAC1.1 and PheSLAC1.2 were able to restore the drought-sensitive phenotype of slac1-3.These results indicated that both PheSLAC1.1 and PheSLAC1.2 played important roles in response to drought stress by regulating stomatal closure.