雪莲口服液影响肠道菌群改善关节炎大鼠关节炎症及细胞因子表达的机制

Study on the mechanism of Saussurea Involucrata Oral Liquid affecting intestinal flora to improve joint inflammation and cytokine expression in arthritis rats

目的:明确雪莲是否可以通过改变大鼠肠道菌群而改善关节炎症及细胞因子。方法:建立Ⅱ型胶原诱导型关节炎(CIA)大鼠模型,经雪莲口服液治疗、肠道菌群衰竭后再治疗、移植肠道菌群(FMT),观察各组大鼠足爪肿胀度,ELISA检测各组炎症因子白细胞介素(IL)-1β、IL-6、IL-10、肿瘤坏死因子-α(TNF-α),HE染色观察滑膜病理,16SrRNA检测肠道菌群。结果:雪莲组与雷公藤组较模型组大鼠血清IL-1β、IL-6显著降低(P<0.05),IL-10、TNF-α显著升高(P<0.01),关节肿胀度减轻(P<0.01),雪莲组肠道菌群较模型组更接近正常组;进行肠道菌群移植后,雪莲粪便组、雷公藤粪便组、正常粪便组以及正常大鼠灌胃雪莲粪便组大鼠关节肿胀度较模型粪便组明显好转(P<0.01),滑膜增生减少,炎症细胞浸润减少。去除肠道菌群干预后,经过雪莲治疗后,雪莲菌群衰竭组大鼠关节炎症较盐水菌群衰竭组差异无统计学意义。结论:雪莲口服液可以通过改变肠道菌群影响炎症因子,改善关节炎症。

Objective:To determine whether Saussurea Involucrata can improve joint inflammation and cytokines by changing intestinal flora in rats.Methods:A rat model of type II collagen-induced arthritis(CIA)was established,treated with Saussurea Involucrata Oral Liquid,treated again after intestinal flora failure,and transplanted intestinal flora(FMT),and the swelling degree of the paws of rats in each group was observed.ELISA was used to detect the inflammatory factors interleukin(IL)-1β,IL-6,IL-10,and tumor necrosis factor-α(TNF-a)in each group,HE staining was used to observe synovial pathology,and 16SrRNA was used to detect intestinal flora.Results:Compared with the model group,IL-1βand IL-6 in the joints of rats in the Saussurea Involucrata group and Tripterygium wilfordii group were decreased(P<0.05),IL-10 and TNF-αwere increased(P<0.01),and joint swelling was reduced(P<0.01).The intestinal flora is closer to the normal group than the model group;after intestinal flora transplantation,the joint swelling of rats in the Saussurea Involucrata feces group,Tripterygium wilfordii feces group,normal feces group,and normal rat gavage Saussurea Involucrata feces group improved(P<0.01),and the synovial proliferation and inflammatory cell infiltration are reduced.After removal of the intestinal flora intervention,and after treatment with Saussurea Involucrata,there was no statistically significant difference in joint inflammation in rats in the Saussurea Involucrata flora depletion group compared with the saline flora depletion group.Conclusion:Saussurea Involucrata Oral Liquid can affect inflammatory factors and improve joint inflammation by changing intestinal flora.