SDS促进复配酶水解排水管网沉积物

SDS enhanced the hydrolysis effect of sediment in drainage pipe network by compound enzyme

采用SDS协同复配酶水解排水管网沉积物,分别考察了SDS投加量、反应时间、pH、温度4个影响因素对沉积物水解效果的影响,并通过SEM、EPS、三维荧光光谱以及粒径等表征方法进行机理分析。结果表明,投加5%(w:w)SDS与8%(w:w)复配酶,在原泥pH(7.28±0.3)、25℃、反应时间为2 h时,沉积物水解效果最好。此时SCOD、多糖和氨氮分别由初始的5686.9、1913.75和87.32 mg·L^(-1)增加到8192.9、3561.29和153.37 mg·L^(-1),有机质含量由56.32%下降到55.59%。在SDS+复配酶处理管网沉积物后,其内部紧实结构被破坏,TB-EPS向S-EPS转移,腐殖酸类物质荧光强度增强,表明溶解性EPS增多,且沉积物粒径向<300μm转变。高通量测序分析结果表明,SDS+复配酶对管网沉积物微生物种类的丰富度和多样性影响不大,但在一定程度上会改变微生物的功能菌群,减少CH4和H2S的产生。以上研究结果可为延缓排水管网淤堵问题提供数据支持。

SDS combined with the compound enzyme were used to hydrolyze the sediment of drainage pipe network.The effects of SDS dosage,reaction time,pH and temperature on the hydrolysis of the sediment were discussed.The mechanism was analyzed by SEM,EPS,3D-EEM and particle size characterization methods.The results showed that the best hydrolysis effect of the sediment happened at 5%(w/w)SDS and 8%(w/w)compound enzyme,pH(7.28±0.3)of raw sediment,25℃and reaction for 2 h.Under these conditions,SCOD,polysaccharide and ammonia nitrogen increased from the initial values of 5686.9mg·L^(-1),1913.75mg·L^(-1) and 87.32mg·L^(-1) to 8192.9,3561.29 and 153.37mg·L^(-1),respectively.Organic matter content decreased from 56.32% to 55.59%.After treated by SDS+compound enzyme,the internal compact structure of sediment was destroyed,TB-EPS transferred to S-EPS,and the fluorescence intensity of humic acids increased,which indicated the increase of the dissolved EPS,and the particle size of sediment decreased to smaller than 300μm.The results of high-throughput sequencing technology showed that SDS+compound enzyme had slight impact on the richness and diversity of microbial species in the sediments,but it could alter the functional microbial community of microorganisms to same extent,and reduced the production of CH4 and H2S.The results of this study provide a new method and data support for delaying the silting problem of the drainage pipe network.